Fluorescence resonance energy transfer detected by scanning near-field optical microscopy

被引：0
作者：
Kirsch, AK
Subramaniam, V
Jenei, A
Jovin, TM
机构：
[1] Max Planck Inst Biophys Chem, Dept Mol Biol, D-37077 Gottingen, Germany
[2] Univ Med Sch Debrecen, Dept Biophys & Cell Biol, Debrecen, Hungary
来源：
JOURNAL OF MICROSCOPY-OXFORD | 1999年 / 194卷
关键词：
cell-surface proteins; fluorescence resonance energy transfer; photobleaching; protein-protein interactions; scanning near-field optical microscopy;
D O I：
暂无
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Fluorescence resonance energy transfer (FRET) between elicited fluorescent donor and acceptor molecules occurs via the Forster mechanism over a range of 1-10 nm. Because of the strong (sixth power) distance dependence of the signal, FRET has been used to assess the proximity of molecules in biological systems. We used a scanning near-field optical microscope (SNOM) operated in the shared-aperture mode using uncoated glass fibre tips to detect FRET between dye molecules embedded in polyvinyl alcohol films and bound to cell surfaces. FRET was detected by selective photobleaching of donor and acceptor fluorophores. We also present preliminary results on pixel-by-pixel energy transfer efficiency measurements using SNOM.
引用
收藏
页码：448 / 454
页数：7
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