Genomic characterization of the complete terpene synthase gene family from Cannabis sativa

被引:47
作者
Allen, Keith D. [1 ,3 ]
McKernan, Kevin [2 ]
Pauli, Christopher [1 ,3 ]
Roe, Jim [1 ,4 ]
Torres, Anthony [1 ,3 ]
Gaudino, Reggie [1 ,3 ]
机构
[1] Steep Hill Labs, Berkeley, CA 94710 USA
[2] Med Genom, Woburn, MA USA
[3] Front Range Biosci, Lafayette, CO USA
[4] Atlas Technol Mfg Co, Port Townsend, WA USA
来源
PLOS ONE | 2019年 / 14卷 / 09期
关键词
LIMONENE SYNTHASE; (+)-ALPHA-PINENE; EXPRESSION; STRINGTIE; REVEALS; MODEL; HISAT;
D O I
10.1371/journal.pone.0222363
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Terpenes are responsible for most or all of the odor and flavor properties of Cannabis sativa, and may also impact effects users experience either directly or indirectly. We report the diversity of terpene profiles across samples bound for the Washington dispensary market. The remarkable degree of variation in terpene profiles ultimately results from action of a family of terpene synthase genes, only some of which have been described. Using a recently available genome assembly we describe 55 terpene synthases with genomic context, and tissue specific expression. The family is quite diverse from a protein similarity perspective, and subsets of the family are expressed in all tissues in the plant, including a set of root specific monoterpene synthases that could well have agronomic importance. Ultimately understanding and breeding for specific terpene profiles will require a good understanding of the gene family that underlies it. We intend for this work to serve as a foundation for that.
引用
收藏
页数:19
相关论文
共 37 条
[1]   Constitutive and induced subterranean plant volatiles attract both entomopathogenic and plant parasitic nematodes [J].
Ali, Jared G. ;
Alborn, Hans T. ;
Stelinski, Lukasz L. .
JOURNAL OF ECOLOGY, 2011, 99 (01) :26-35
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   Genomic analysis of the terpenoid synthase (AtTPS) gene family of Arabidopsis thaliana [J].
Aubourg, S ;
Lecharny, A ;
Bohlmann, J .
MOLECULAR GENETICS AND GENOMICS, 2002, 267 (06) :730-745
[4]   Plant terpenoid synthases: Molecular biology and phylogenetic analysis [J].
Bohlmann, J ;
Meyer-Gauen, G ;
Croteau, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (08) :4126-4133
[5]   Terpene synthases from Cannabis sativa [J].
Booth, Judith K. ;
Page, Jonathan E. ;
Bohlmann, Jorg .
PLOS ONE, 2017, 12 (03)
[6]  
Brenneisen Rudolf, 2007, P17, DOI 10.1007/978-1-59259-947-9_2
[7]   Annotation of the Corymbia terpene synthase gene family shows broad conservation but dynamic evolution of physical clusters relative to Eucalyptus [J].
Butler, Jakob B. ;
Freeman, Jules S. ;
Potts, Brad M. ;
Vaillancourt, Rene E. ;
Grattapaglia, Dario ;
Silva-Junior, Orzenil B. ;
Simmons, Blake A. ;
Healey, Adam L. ;
Schmutz, Jeremy ;
Barry, Kerrie W. ;
Lee, David J. ;
Henry, Robert J. ;
King, Graham J. ;
Baten, Abdul ;
Shepherd, Mervyn .
HEREDITY, 2018, 121 (01) :87-104
[8]   The family of terpene synthases in plants: a mid-size family of genes for specialized metabolism that is highly diversified throughout the kingdom [J].
Chen, Feng ;
Tholl, Dorothea ;
Bohlmann, Joerg ;
Pichersky, Eran .
PLANT JOURNAL, 2011, 66 (01) :212-229
[9]   Monoterpene and sesquiterpene synthases and the origin of terpene skeletal diversity in plants [J].
Degenhardt, Joerg ;
Koellner, Tobias G. ;
Gershenzon, Jonathan .
PHYTOCHEMISTRY, 2009, 70 (15-16) :1621-1637
[10]   Product Variability of the 'Cineole Cassette' Monoterpene Synthases of Related Nicotiana Species [J].
Faehnrich, Anke ;
Krause, Katrin ;
Piechulla, Birgit .
MOLECULAR PLANT, 2011, 4 (06) :965-984