Chemical characteristic of an anticoagulant-active sulfated polysaccharide from Enteromorpha clathrata

被引：136
作者：
Qi, Xiaohui [1 ]
Mao, Wenjun [1 ]
Gao, Yan [2 ]
Chen, Yin [1 ]
Chen, Yanli [1 ]
Zhao, Chunqi [1 ]
Li, Na [1 ]
Wang, Chunyan [1 ]
Yan, Mengxia [1 ]
Lin, Cong [1 ]
Shan, Jimiao [1 ]
机构：
[1] Ocean Univ China, Inst Marine Drug & Food, Minist Educ, Key Lab Marine Drugs, Qingdao 266003, Peoples R China
[2] Natl Oceanog Ctr Qingdao, Qingdao 266071, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2012年 / 90卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Enteromorpha clathrata; Sulfated polysaccharide; Chemical characteristic; Anticoagulant activity; HEPARIN-COFACTOR-II; ANTIOXIDANT PROPERTIES; GREEN-ALGA; GALACTAN;
D O I：
10.1016/j.carbpol.2012.07.077
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A sulfated polysaccharide FEP from marine green alga Enteromorpha clathrata was extracted with hot water and further purified by ion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that FEP was a high arabinose-containing sulfated polysaccharide with sulfate ester of 31.0%, and its average molecular weight was about 511 kDa. The backbone of FEP was mainly composed of (1 -> 4)-linked beta-L-arabinopyranose residues with partially sulfate groups at the C-3 position. In vitro anticoagulant assay indicated that FEP effectively prolonged the activated partial thromboplastin time and thrombin time. The investigation demonstrated that FEP was a novel sulfated polysaccharide with different chemical characteristics from other sulfated polysaccharides from marine algae, and could be a potential source of anticoagulant. (C) 2012 Elsevier Ltd. All rights reserved,
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页码：1804 / 1810
页数：7
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