Research on Gene Introgression from Common Wheat into Aegilops L

We carried out a group of experiments to verify possibility of gene introgression from common wheat into Aegilops. The artificial indoor crossbreed was conducted using 7 genotypes from 4 wheat relative species as female and common wheat as male. The experiment result shows that different species have variable cross ability. Among the 4 Aegilops species, the highest cross rate is from the combination of Ae. tauschii T. aestivum 46.49% for genotype Ae42, 22.58% for Y92), the second is from Ae. ovata square T. astivum 14.76% for Y100, 12.11% for Ae23), the third is from Ae. cylindrica T. astivum 2.23% for Ae7, 8.50% for Y145), the lowest is from Ae. speltoides square T. astivum 0.19%). Hybrid embryos from different combinations have different abilities of callus initiation and germination. The hybrid embryos from A. ovata / T. eastivum and Ae. tauschii / T. eastivum have higher level of callus initiation and germination, Ae. cylindrica / T. eastivum has middle level, while the Ae. speltoides has lower level. The interspecific hybrids between Aegilops and common wheat have so low fertility. In back-crosses, the seed-set rate of hybrids of Ae. ovata / T. aestivum is 3.71% and 4.36% respectively back-crossed with male and female parents while for hybrids of A. cylindrica / T. aestivum, it is 0 and 0.33% respectively and for A. tauschii / T. aestivum, 0.33% and 0 respectively. On selfing of the hybrids, the seed-set rate is 0 no seed set from 9750 florets) for the combination of Ae. cylindrica / T. aestivum, 0.044% 3 selfed seeds out of 6870 florets) for A. ovata / T. aestivum and 0 no seed set from 7253 florets) for A. tauschii / T. aestivum. The research suggested that the probability of gene introgression from T. aestivum into Aegilops species is very low in nature.