Configurational Biomimetic Imprinting for Protein Recognition: Structural Characteristics of Recognitive Hydrogels

被引：27

作者：

Bergmann, Nicole M. ^{[1
,2
]}

Peppas, Nicholas A. ^{[1
]}

机构：

[1] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

[2] Univ Texas Austin, Dept Chem Engn, Austin, TX 78712 USA

来源：

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH | 2008年 / 47卷 / 23期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1021/ie071270u

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

Using a configurational biomimetic imprinting technique, we prepared a family of molecularly imprinting polyacrylamide polymers with recognition for the template protein chicken egg white lysozyme in aqueous solution. We showed the formation of a network with specific recognitive sites for the template. To determine the overall macroscopic properties of protein-imprinted gel, we utilized a variety of tools. When gels were examined using scanning electron microscopy, definite morphological differences were observed between the CBIP polymers and the controls. Additionally, Fourier transform infrared spectroscopy determined that the presence of template had no effect upon the overall gel composition, whereas differential scanning calorimetry showed no significant change in the molecular weight between cross-links between CBIP polymers and nonimprinted polymers. Using these results, a theory of gel formation was proposed to try to elucidate gel formation in the presence of a macromolecule.

引用

页码：9099 / 9107

页数：9

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