Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell

被引：12

作者：

Xu, Jian ^{[1
]}

Mon, Hiroaki ^{[1
]}

Kusakabe, Takahiro ^{[1
]}

Li, Zhiqing ^{[1
]}

Zhu, Li ^{[1
]}

Iiyama, Kazuhiro ^{[2
]}

Masuda, Atsushi ^{[1
]}

Mitsudome, Takumi ^{[1
]}

Lee, Jae Man ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Lab Silkworm Sci, Higashi Ku, Fukuoka 8128581, Japan

[2] Kyushu Univ, Fac Agr,Grad Sch,Inst Biol Control, Lab Insect Pathol & Microbial Control, Higashi Ku, Fukuoka 8128581, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2013年 / 97卷 / 24期

基金：

日本学术振兴会;

关键词：

RNA interference; SID-1; Bme21; BEVS; Baculovirus; BETA-N-ACETYLGLUCOSAMINIDASE; DOUBLE-STRANDED-RNA; LOBES GENE ENCODES; ECTOPIC EXPRESSION; PROTEIN; SILKWORM; DSRNA; SF9; IDENTIFICATION; TRANSPORT;

D O I：

10.1007/s00253-013-5279-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The double-stranded RNA (dsRNA) mediated RNA interference (RNAi) is widely employed in silkworm and its tissue-derived cell lines for gene function analysis. Baculovirus expression vector system (BEVS) has an advantage for large-scale protein expression. Previously, combining these useful tools, we improved traditional AcMNPV-Sf9 BEVS to produce modified target glycoproteins, where the ectopic expression of Caenorhabditis elegans systemic RNAi defective-1 (SID-1) was found to be valuable for soaking RNAi. In current study, we applied CeSID-1 protein to a Bombyx mori NPV (BmNPV)-hypersensitive Bme21 cell line and investigated its properties both in soaking RNAi ability and recombinant protein expression. The soaking RNAi-mediated suppression in the Bme21 cell enables us to produce modified glycoproteins of interest in BmNPV-Bme21 BEVS.

引用

页码：10435 / 10444

页数：10

共 37 条

[1] INSECT CELLS CONTAIN AN UNUSUAL, MEMBRANE-BOUND BETA-N-ACETYLGLUCOSAMINIDASE PROBABLY INVOLVED IN THE PROCESSING OF PROTEIN N-GLYCANS [J].