Systemic availability and metabolism of colonic-derived short-chain fatty acids in healthy subjects: a stable isotope study

被引：293

作者：

Boets, Eef ^{[1
,2
]}

Gomand, Sara V. ^{[2
,3
]}

Deroover, Lise ^{[1
,2
]}

Preston, Tom ^{[5
]}

Vermeulen, Karen ^{[6
]}

De Preter, Vicky ^{[1
,7
]}

Hamer, Henrike M. ^{[1
,2
]}

Van den Mooter, Guy ^{[4
]}

De Vuyst, Luc ^{[8
]}

Courtin, Christophe M. ^{[2
,3
]}

Annaert, Pieter ^{[4
]}

Delcour, Jan A. ^{[2
,3
]}

Verbeke, Kristin A. ^{[1
,2
]}

机构：

[1] Katholieke Univ Leuven, Translat Res Gastrointestinal Disorders, Leuven, Belgium

[2] Katholieke Univ Leuven, Leuven Food Sci & Nutr Res Ctr, Leuven, Belgium

[3] Katholieke Univ Leuven, Ctr Food & Microbial Technol, Leuven, Belgium

[4] Katholieke Univ Leuven, Drug Delivery & Disposit, Leuven, Belgium

[5] Univ Glasgow, Stable Isotope Biochem Lab, Scottish Univ Environm Res Ctr, Glasgow, Lanark, Scotland

[6] Univ Ghent, Dept Pathol Bacteriol & Avian Dis, Merelbeke, Belgium

[7] UC Leuven Limburg, Grp Hlth & Social Work, Leuven, Belgium

[8] Vrije Univ Brussel, Ind Microbiol & Food Biotechnol, Brussels, Belgium

来源：

JOURNAL OF PHYSIOLOGY-LONDON | 2017年 / 595卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

colonic fermentation; metabolism; short-chain fatty acids; stable isotopes; systemic exposure;

D O I：

10.1113/JP272613

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Key points: The short-chain fatty acids (SCFAs) are bacterial metabolites produced during the colonic fermentation of undigested carbohydrates, such as dietary fibre and prebiotics, and can mediate the interaction between the diet, the microbiota and the host. We quantified the fraction of colonic administered SCFAs that could be recovered in the systemic circulation, the fraction that was excreted via the breath and urine, and the fraction that was used as a precursor for glucose, cholesterol and fatty acids. This information is essential for understanding the molecular mechanisms by which SCFAs beneficially affect physiological functions such as glucose and lipid metabolism and immune function. Abstract: The short-chain fatty acids (SCFAs), acetate, propionate and butyrate, are bacterial metabolites that mediate the interaction between the diet, the microbiota and the host. In the present study, the systemic availability of SCFAs and their incorporation into biologically relevant molecules was quantified. Known amounts of 13C-labelled acetate, propionate and butyrate were introduced in the colon of 12 healthy subjects using colon delivery capsules and plasma levels of 13C-SCFAs 13C-glucose, 13C-cholesterol and 13C-fatty acids were measured. The butyrate-producing capacity of the intestinal microbiota was also quantified. Systemic availability of colonic-administered acetate, propionate and butyrate was 36%, 9% and 2%, respectively. Conversion of acetate into butyrate (24%) was the most prevalent interconversion by the colonic microbiota and was not related to the butyrate-producing capacity in the faecal samples. Less than 1% of administered acetate was incorporated into cholesterol and <15% in fatty acids. On average, 6% of colonic propionate was incorporated into glucose. The SCFAs were mainly excreted via the lungs after oxidation to 13CO2, whereas less than 0.05% of the SCFAs were excreted into urine. These results will allow future evaluation and quantification of SCFA production from 13C-labelled fibres in the human colon by measurement of 13C-labelled SCFA concentrations in blood. © 2016 The Authors. The Journal of Physiology © 2016 The Physiological Society

引用

页码：541 / 541

页数：1

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