Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins

被引：63

作者：

Butkevich, Alexey N. ^{[1
]}

Ta, Haisen ^{[1
]}

Ratz, Michael ^{[1
,3
]}

Stoldt, Stefan ^{[1
]}

Jakobs, Stefan ^{[1
,2
]}

Belov, Vladimir N. ^{[1
]}

Hell, Stefan W. ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept Nanobiophoton, Fassberg 11, D-37077 Gottingen, Germany

[2] Univ Gottingen, Dept Neurol, Med Fac, Robert Koch Str 40, D-37075 Gottingen, Germany

[3] Karolinska Inst, Dept Cell & Mol Biol, SE-17177 Stockholm, Sweden

来源：

ACS CHEMICAL BIOLOGY | 2018年 / 13卷 / 02期

关键词：

EMISSION DEPLETION NANOSCOPY; NEAR-INFRARED FLUOROPHORE; SUPERRESOLUTION MICROSCOPY; LIVE-CELL; FLUORESCENT DYES; RHODAMINE; BIOLOGY; PROBES; RED;

D O I：

10.1021/acschembio.7b00616

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.

引用

页码：475 / 480

页数：6

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