Nanopolyaniline as Immobilization Template for Signal Enhancement of Surface Plasmon Resonance Biosensor - A Preliminary Study

被引：2

作者：

Kamarun, Dzaraini ^{[1
]}

Azem, Nor Hazirah Kamel Abdul ^{[1
]}

Sarijo, Siti Halimah ^{[1
]}

Mohd, Ahmad Faiza ^{[1
]}

Noor, Mashita Abdullah Mohd ^{[1
]}

机构：

[1] Univ Teknol MARA UiTM, Fac Sci Appl, Shah Alam 40450, Selangor Darul, Malaysia

来源：

2ND ASEAN - APCTP WORKSHOP ON ADVANCED MATERIALS SCIENCE AND NANOTECHNOLOGY (AMSN 2010) | 2012年 / 1455卷

关键词：

Surface Plasmon Resonance; biomolecular interactions; immobilization; immunosensor; biosensor; POLYANILINE NANOPARTICLES; POLYMERIZATION;

D O I：

10.1063/1.4732472

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A technique for the enhancement of Surface Plasmon Resonance (SPR) signal for sensing biomolecular interactions is described. Polyaniline (PANI) of particle size in the range of 1 to 15 nm was synthesized and used as the template for the immobilization of protein molecules. Biomolecular interactions of unbound and PANI-bound proteins with antibody molecules were SPR-monitored using a model system comprising of Bovine Serum Albumin (BSA) and antiBSA. A 7-fold increased in the signal was recorded from interactions of the PANI-bound BSA with antiBSA compared to the interactions of its unbound counterpart. This preliminary observation provides new avenue in immunosensor technology for improving the detection sensitivity of SPR biosensor; and thereby increasing the lower detection limit of biomolecules.

引用

页码：60 / 69

页数：10

共 21 条

[1] Polyaniline by new miniemulsion polymerization and the effect of reducing agent on conductivity [J].

Bhadra, Sambhu ;

Singha, Nikhil K. ;

Khastgir, Dipak .

SYNTHETIC METALS, 2006, 156 (16-17) :1148-1154

[2] The design, synthesis and characterization of polyaniline nanophase materials [J].

Chen, Jiangfeng ;

Xu, Yiting ;

Zheng, Yifang ;

Dai, Lizong ;

Wu, Huihuang .

COMPTES RENDUS CHIMIE, 2008, 11 (1-2) :84-89

[3] Optical properties of biocompatible polyaniline nano-composites [J].

Dispenza, C. ;

Leone, M. ;

Lo Presti, C. ;

Librizzi, F. ;

Spadaro, G. ;

Vetri, V. .

JOURNAL OF NON-CRYSTALLINE SOLIDS, 2006, 352 (36-37) :3835-3840

[4] Aqueous/ionic liquid interfacial polymerization for preparing polyaniline nanoparticles [J].

Gao, HX ;

Jiang, T ;

Han, BX ;

Wang, Y ;

Du, JM ;

Liu, ZM ;

Zhang, JL .

POLYMER, 2004, 45 (09) :3017-3019

[5] POLYMER MATERIALS IN BIOSENSORS [J].

GECKELER, KE ;

MULLER, B .

NATURWISSENSCHAFTEN, 1993, 80 (01) :18-24

[6] Monitoring RNA transcription in real time by using surface plasmon resonance [J].

Greive, Sandra J. ;

Weitzel, Steven E. ;

Goodarzi, Jim P. ;

Main, Lisa J. ;

Pasman, Zvi ;

von Hippel, Peter H. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (09) :3315-3320

[7] Synthesis of polyaniline/multi-walled carbon nanotube nanocomposites in water/oil microemulsion [J].

Guo, Hongfan ;

Zhu, Hong ;

Lin, Haiyan ;

Zhang, Jiqiao .

MATERIALS LETTERS, 2008, 62 (24) :3919-3921

[8] Enhanced permeability of polyaniline based nano-membranes for gas separation [J].

Gupta, Y. ;

Hellgardt, K. ;

Wakeman, R. J. .

JOURNAL OF MEMBRANE SCIENCE, 2006, 282 (1-2) :60-70

[9] A general chemical route to polyaniline nanofibers [J].

Huang, JX ;

Kaner, RB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (03) :851-855

[10]

JOHNSSON B, 1995, J MOL RECOGNIT, V8, P125, DOI 10.1002/jmr.300080122

← 1 2 3 →