In Vitro Osteoclast Differentiation of Bone Marrow Cells on Hydroxyapatite/Collagen Self-Organized Bone-Like Nanocomposite Disk

被引：4
作者：
Kikuchi, Masanori [1 ]
Irie, Atsushi [2 ]
机构：
[1] Natl Inst Mat Sci, Ctr Biomat, 1-1 Namiki, Tsukuba, Ibaraki 3050044, Japan
[2] Tokyo Metropolitan Inst Med Sci, Biomembrane Signaling Project, Bunkyo Ku, Tokyo 113, Japan
来源：
BIOCERAMICS 21 | 2009年 / 396-398卷
关键词：
Hydroxyapatite; collagen; nanocomposite; osteoclast; differentiation; OSTEOGENIC ACTIVITY; STROMAL CELLS; MG63; CELLS; PHOSPHATE; MEMBRANE; CULTURE;
D O I：
10.4028/www.scientific.net/KEM.396-398.449
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Osteoclast differentiation of bone marrow cells on hydroxyapatite/collagen self-organized bone-like nanocomposite (HAp/Col) disk in vitro was evaluated by coculture of mouse bone marrow cells with mouse osteoblasts with or without addition of osteoclastic inducers, 1,25-Dihydroxyvitamin D-3 and 1 mu M prostaglandin E-2. Dentine slice and tissue culture polystyrene were used as controls. Good osteoclastic differentiation at day 7 were observed among the bone marrow cells cultured on the HAp/Col disk and controls with osteoclastic inducers. On the contrary, osteoclastic differentiation was observed only for marrow cells Cultured on the HAp/Col disk. Nano- and micro- structures as well as chemical and mechanical properties have a potential to control cell differentiation.
引用
收藏
页码：449 / 452
页数：4
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