Leaves of olive (Olea europaea var. minor rotunda) were collected from mature non-irrigated trees in an "off" year, at monthly intervals, from May 2015 to April 2016. Leaf concentrations of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn) and boron (B) were determined. Seasonal fluctuation curves were developed for each nutrient, in order to use them for interpretation of leaf analysis. Concentrations of N, P, K, Ca and Mg showed significant fluctuation throughout the year, while B, Fe, Mn and Zn showed less intense changes. Nutrient accumulation varied according to tree vegetative growth and weather conditions.