Identification of respiratory isolates of Stenotrophomonas maltophilia by commercial biochemical systems and species-specific PCR

被引:5
作者
Giordano, A
Magni, A
Trancassini, M
Varesi, P
Turner, R
Mancini, C
机构
[1] Univ Roma La Sapienza, Dipartimento Sci Sanita Pubbl, Sez Microbiol, I-00136 Rome, Italy
[2] BioMerieux Italia, Rome, Italy
关键词
identification; biochemical; molecular; Stenotrophomonas maltophilia;
D O I
10.1016/j.mimet.2005.04.020
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
One hundred strains of Stenotrophomonas maltophilia isolates from respiratory specimens were biochemically identified using the API 20NE strip and the VITEK (R) 2 ID-GNB card. The identification was confirmed by a species-specific PCR using two primers specific for the 23S rRNA gene. The API 20NE showed only I strain with "low discrimination" whereas the VITEK (R) 2 gave 12. In any case.. the two biochemical systems showed good reliability compared to SS-PCR. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:135 / 138
页数:4
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