Fast nucleic acid amplification for integration in point-of-care applications

被引：21

作者：

Brunklaus, Sabine ^{[1
]}

Hansen-Hagge, Thomas E. ^{[2
]}

Erwes, Julia ^{[1
]}

Hoeth, Julian ^{[1
]}

Jung, Mathieu ^{[1
]}

Latta, Daniel ^{[1
]}

Strobach, Xenia ^{[1
]}

Winkler, Christian ^{[1
]}

Ritzi-Lehnert, Marion ^{[1
]}

Drese, Klaus S. ^{[1
]}

机构：

[1] Inst Mikrotech Mainz GmbH, D-55129 Mainz, Germany

[2] Microfluid ChipShop, Jena, Germany

来源：

ELECTROPHORESIS | 2012年 / 33卷 / 21期

关键词：

Lab-on-a-chip; Microfluidics; PCR; POLYMERASE-CHAIN-REACTION; PCR; DNA;

D O I：

10.1002/elps.201200259

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An ultrafast microfluidic PCR module (30 PCR cycles in 6 min) based on the oscillating fluid plug concept was developed. A robust amplification of native genomic DNA from whole blood samples could be achieved at operational conditions established from systematic investigations of key parameters including heat transfer and in particular flow velocities. Experimental data were augmented with results from computational fluid dynamics simulations. The reproducibility of the current system was substantially improved compared to previous concepts by integration of a closed reservoir instead of utilizing a vented channel end at ambient pressure rendering the devised module suitable for integration into complex sample-to-answer analysis platforms such as point-of-care applications.

引用

页码：3222 / 3228

页数：7

共 30 条

[1] Miniaturised nucleic acid analysis [J].