Effects of choline on the phenotype of the cultured bovine preimplantation embryo

被引:16
作者
Estrada-Cortes, E. [1 ,2 ,3 ]
Negron-Perez, V. M. [4 ]
Tribulo, P. [5 ,6 ,7 ,8 ]
Zenobi, M. G. [5 ,6 ]
Staples, C. R. [1 ,2 ]
Hansen, P. J. [1 ,2 ]
机构
[1] Univ Florida, Dept Anim Sci, DH Barron Reprod & Perinatal Biol Res Program, Gainesville, FL 32611 USA
[2] Univ Florida, Genet Inst, Gainesville, FL 32611 USA
[3] Inst Nacl Invest Forestales Agr & Pecuarias, Campo Expt Ctr Altos de Jalisco, Tepatitlan De Morelos 47600, Jalisco, Mexico
[4] Univ Puerto Rico MayagUez, Dept Anim Sci, Mayaguez, PR 00681 USA
[5] Inst Reprod Anim Cordoba, X5145, Cordoba, Argentina
[6] Consejo Nacl Invest Cient & Tecn, X5145, Cordoba, Argentina
[7] Univ Nacl Cordoba, Fac Ciencias Agr, X5000, Cordoba, Argentina
[8] Consejo Nacl Invest Cient & Tecn, X5000, Cordoba, Argentina
基金
美国国家卫生研究院;
关键词
choline; embryo; blastocyst; gene expression; DNA methylation; PROTECTED CHOLINE; KINASE-ALPHA; METHIONINE; EXPRESSION; SUPPLEMENTATION; GENE; PHOSPHATIDYLCHOLINE; IDENTIFICATION; METHYLATION; PERFORMANCE;
D O I
10.3168/jds.2020-18598
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Choline is a precursor of acetylcholine, phosphatidylcholine, and the methyl-donor betaine. Reports indicate that supplementation with rumen-protected choline improves postpartum reproductive function of dairy cows. The objective was to determine whether addition of choline to culture medium of in vitro-produced embryos alters the phenotype of the resultant blastocysts. Treat-ments were choline chloride (ChCl; 0.004, 1.3, 1.8, and 6.37 mM) and phosphatidylcholine (1.3 mM). Treatment with 0.004 mM ChCl improved development to the blastocyst stage, increased blastocyst cell number, and increased the percentage of blastocysts that were hatching or hatched. Development was not affected by higher concentrations of ChCl but was reduced by 1.3 mM phosphatidylcholine. Treatment of embryos with 1.3 mM ChCl (but not other concentrations) increased expression in blastocysts of 11 of 165 genes examined (AMOT, NANOG, HDAC8, HNF4A, STAT1, MBNL3, SOX2, STAT3, KDM2B, SAV1, and GPAM) and decreased expression of one gene (ASS1). Treatment with 1.3 mM ChCl decreased global DNA methylation at d 3.5 of development and increased DNA methylation at d 7.5 in blastocysts. Treatment with 1.8 mM ChCl also increased methylation in blastocysts. In conclusion, addition of choline to the culture medium alters the phenotype of preimplantation bovine embryos produced in vitro. Choline chloride can act in a concentration-dependent manner to alter development, expression of specific genes, and DNA methylation.
引用
收藏
页码:10784 / 10796
页数:13
相关论文
共 45 条
[1]   Effects of rumen-protected methionine and choline supplementation on the preimplantation embryo in Holstein cows [J].
Acosta, D. A. V. ;
Denicol, A. C. ;
Tribulo, P. ;
Rivelli, M. I. ;
Skenandore, C. ;
Zhou, Z. ;
Luchini, D. ;
Correa, M. N. ;
Hansen, P. J. ;
Cardoso, F. C. .
THERIOGENOLOGY, 2016, 85 (09) :1669-1679
[2]   Effect of rumen-protected choline and methionine on physiological and metabolic disorders and reproductive indices of dairy cows [J].
Ardalan, M. ;
Rezayazdi, K. ;
Dehghan-Banadaky, M. .
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, 2010, 94 (06) :E259-E265
[3]   Effect of injection of β-carotene or vitamin E and selenium on fertility of lactating dairy cows [J].
Arechiga, CF ;
Vázquez-Flores, S ;
Ortíz, O ;
Hernández-Cerón, J ;
Porras, A ;
McDowell, LR ;
Hansen, PJ .
THERIOGENOLOGY, 1998, 50 (01) :65-76
[4]   Choline and Choline Metabolite Patterns and Associations in Blood and Milk during Lactation in Dairy Cows [J].
Artegoitia, Virginia M. ;
Middleton, Jesse L. ;
Harte, Federico M. ;
Campagna, Shawn R. ;
de Veth, Michael J. .
PLOS ONE, 2014, 9 (08)
[5]   Phospholipid Profile and Distribution in the Receptive Oviduct and Uterus During Early Diestrus in Cattle [J].
Belaz, Katia Roberta A. ;
Tata, Alessandra ;
Franca, Moana R. ;
Santos da Silva, Maressa I. ;
Vendramini, Pedro Henrique ;
Fernandes, Anna Maria A. P. ;
D'Alexandri, Fabio L. ;
Eberlin, Marcos N. ;
Binelli, Mario .
BIOLOGY OF REPRODUCTION, 2016, 95 (06) :1-11
[6]   Efficacy of in vitro embryo transfer in lactating dairy cows using fresh or vitrified embryos produced in a novel embryo culture medium [J].
Block, J. ;
Bonilla, L. ;
Hansen, P. J. .
JOURNAL OF DAIRY SCIENCE, 2010, 93 (11) :5234-5242
[7]   Methionine Requirements for the Preimplantation Bovine Embryo [J].
Bonilla, Luciano ;
Luchini, Daniel ;
Devillard, Estelle ;
Hansen, Peter J. .
JOURNAL OF REPRODUCTION AND DEVELOPMENT, 2010, 56 (05) :527-532
[8]   Nutritional interactions with reproductive performance in dairy cattle [J].
Butler, WR .
ANIMAL REPRODUCTION SCIENCE, 2000, 60 :449-457
[9]   The embryonic stress response to in vitro culture: insight from genomic analysis [J].
Cagnone, Gael ;
Sirard, Marc-Andre .
REPRODUCTION, 2016, 152 (06) :R247-R261
[10]   Identification of a Physiologically Relevant Endogenous Ligand for PPARα in Liver [J].
Chakravarthy, Manu V. ;
Lodhi, Irfan J. ;
Yin, Li ;
Malapaka, Raghu R. V. ;
Xu, H. Eric ;
Turk, John ;
Semenkovich, Clay F. .
CELL, 2009, 138 (03) :476-488