Surface-enhanced Raman scattering study of carcinoembryonic antigen in serum from patients with colorectal cancers

被引：10
作者：
Chen, Gang [1 ,2 ]
Chen, Yanping [1 ]
Zheng, Xiongwei [1 ]
He, Cheng [1 ]
Lu, Jianping [1 ]
Feng, Shangyuan [3 ]
Chen, Rong [3 ]
Zeng, Haisan [4 ]
机构：
[1] Fujian Med Univ, Teaching Hosp, Fujian Prov Tumor Hosp, Dept Pathol, Fuzhou 350014, Peoples R China
[2] Fujian Prov Key Lab Translat Canc Med, Fuzhou 350014, Peoples R China
[3] Fujian Normal Univ, Minist Educ, Key Lab OptoElect Sci & Technol Med, Fuzhou 350007, Peoples R China
[4] British Columbia Canc Agcy Res Ctr, Integrat Oncol Dept, Imaging Unit, Vancouver, BC V5Z 1L3, Canada
来源：
APPLIED PHYSICS B-LASERS AND OPTICS | 2013年 / 113卷 / 04期
基金：
加拿大健康研究院; 中国国家自然科学基金;
关键词：
SELF-ASSEMBLED MONOLAYER; BIOANALYTICAL APPLICATIONS; HOMOGENEOUS DETECTION; NANOPARTICLES; IMMUNOASSAY; DNA; SPECTROSCOPY; AGGREGATION; MOLECULE; CELLS;
D O I：
10.1007/s00340-013-5515-1
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
In this work, we developed a SERS platform for quantitative detection of carcinoembryonic antigen (CEA) in serum of patients with colorectal cancers. Anti-CEA-functionalized 4-mercaptobenzoic acid-labeled Au/Ag core-shell bimetallic nanoparticles were prepared first and then used to analyze CEA antigen solutions of different concentrations. A calibration curve was established in the range from 5 x 10(-3) to 5 x 10(5) ng/mL. Finally, this new SERS probe was applied for quantitative detection of CEA in serum obtained from 26 colorectal cancer patients according to the calibration curve. The results were in good agreement with that obtained by electrochemical luminescence method, suggesting that SERS immunoassay has high sensitivity and specificity for CEA detection in serum. A detection limit of 5 pg/ml was achieved. This study demonstrated the feasibility and great potential for developing this new technology into a clinical tool for analysis of tumor markers in the blood.
引用
收藏
页码：597 / 602
页数：6
相关论文
共 35 条
[1]   SERS-Based Diagnosis and Biodetection [J].
Alvarez-Puebla, Ramon A. ;
Liz-Marzan, Luis M. .
SMALL, 2010, 6 (05) :604-610
[2]  
[Anonymous], CA CANC J CLIN, DOI DOI 10.3322/CAAC.20107
[3]   Recent progress in SERS biosensing [J].
Bantz, Kyle C. ;
Meyer, Audrey F. ;
Wittenberg, Nathan J. ;
Im, Hyungsoon ;
Kurtulus, Ozge ;
Lee, Si Hoon ;
Lindquist, Nathan C. ;
Oh, Sang-Hyun ;
Haynes, Christy L. .
PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2011, 13 (24) :11551-11567
[4]   Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection [J].
Cao, YWC ;
Jin, RC ;
Mirkin, CA .
SCIENCE, 2002, 297 (5586) :1536-1540
[5]   International Trends in Colorectal Cancer Incidence Rates [J].
Center, Melissa M. ;
Jemal, Ahmedin ;
Ward, Elizabeth .
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 2009, 18 (06) :1688-1694
[6]   Immunoassay using surface-enhanced Raman scattering based on aggregation of reporter-labeled immunogold nanoparticles [J].
Chen, Ji-Wei ;
Lei, Yong ;
Liu, Xiang-Jiang ;
Jiang, Jian-Hui ;
Shen, Guo-Li ;
Yu, Ru-Qin .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 392 (1-2) :187-193
[7]   Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering [J].
Chen, Yanping ;
Zheng, Xiongwei ;
Chen, Gang ;
He, Chen ;
Zhu, Weifeng ;
Feng, Shangyuan ;
Xi, Gangqin ;
Chen, Rong ;
Lan, Fenghua ;
Zeng, Haishan .
INTERNATIONAL JOURNAL OF NANOMEDICINE, 2012, 7 :73-82
[8]   Covalent attachment of synthetic DNA to self-assembled monolayer films [J].
Chrisey, LA ;
Lee, GU ;
OFerrall, CE .
NUCLEIC ACIDS RESEARCH, 1996, 24 (15) :3031-3039
[9]   Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics [J].
Culha, M ;
Stokes, D ;
Allain, LR ;
Vo-Dinh, T .
ANALYTICAL CHEMISTRY, 2003, 75 (22) :6196-6201
[10]   Homogeneous immunoassay based on aggregation of antibody-functionalized gold nanoparticles coupled with light scattering detection [J].
Du, Baoan ;
Li, Zhengping ;
Cheng, Yongqiang .
TALANTA, 2008, 75 (04) :959-964
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