Characterization of Markers of the Progression of Human Parvovirus B19 Infection in Virus DNA-Positive Plasma Samples

被引：6

作者：

Bonjoch, Xavier ^{[1
]}

Obispo, Francesc ^{[1
]}

Alemany, Cristina ^{[1
]}

Pacha, Ana ^{[1
]}

Rodriguez, Esteban ^{[1
]}

Xairo, Dolors ^{[1
]}

机构：

[1] Biomat SA, Grifols, Div Anal, Barcelona 08150, Spain

来源：

TRANSFUSION MEDICINE AND HEMOTHERAPY | 2015年 / 42卷 / 04期

关键词：

Antibodies; Blood safety; Donation deferral period; Immune response; Parvovirus B19; Plasma donation; Transfusion-associated infections; POLYMERASE-CHAIN-REACTION; HUMAN ERYTHROVIRUS B19; SICKLE-CELL-DISEASE; DRY-HEAT-TREATMENT; RED-BLOOD-CELLS; REAL-TIME PCR; NONENVELOPED VIRUSES; DONORS; PREVALENCE; VIREMIA;

D O I：

10.1159/000381979

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Accurate characterization of the infection stage in parvovirus B19(B19V)-positive plasma donations would help establish the donation deferral period to contribute to a safe fractionation pool of plasma. Methods: Viral DNA load of 74 B19V DNA-positive plasma samples from whole blood donations was determined by titration using nucleic acid testing. Markers of cellular (neopterin) and humoral (B19V-specific IgM and IgG) immune response were determined by ELISA in 32 B19V DNA-positive samples and in 13 B19V DNA-negative samples. The infection progression profile was estimated according to B19V DNA load and the presence of immune response markers. Results: B19V DNA load in the 74 samples was 10(6)-10(13) IU/ml. The distribution of 14 out of 32 selected B19V DNA-positive samples plus 2 B19V DNA-negative samples with no immune response marker followed along an upward curve according to B19V DNA load. After the peak, the distribution of 18 immune marker-positive samples followed along a downward curve according to their B19V DNA load and was grouped as follows: neopterin In = 4), neopterin+ IgM (n = 8), neopterin + IgM + IgG In = 3), IgM + IgG (n = 2), IgM (n = 1). There were 11 B19V DNA-negative IgG-positive samples. Conclusion: This study of B19V-DNA load and levels of neopterin, IgM, and IgG allows for reliable characterization and distribution into the different stages of B19V infection. (C) 2015 S. Karger GmbH, Freiburg

引用

页码：233 / 238

页数：8

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