Human Seminal Plasma Fosters CD4+Regulatory T-cell Phenotype and Transforming Growth Factor-ß1 Expression

被引：25

作者：

Balandya, Emmanuel ^{[1
]}

Wieland-Alter, Wendy ^{[1
]}

Sanders, Katherine ^{[2
]}

Lahey, Timothy ^{[1
]}

机构：

[1] Dartmouth, Geisel Sch Med, Lebanon, NH 03756 USA

[2] Dartmouth Coll, Hanover, NH 03755 USA

来源：

AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2012年 / 68卷 / 04期

关键词：

FoxP3; seminal plasma; TGF-ss; Treg; REGULATORY-CELLS; IMMUNE TOLERANCE; DRIVES EXPANSION; CD4(+)CD25(+); INDUCTION; CONVERSION; FOXP3; SEMEN; INFERTILITY; MECHANISMS;

D O I：

10.1111/j.1600-0897.2012.01176.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Problem Semen mediates expansion of CD4+ regulatory T cells (Treg) in the murine female reproductive tract. The impact of semen on Treg in humans, however, remains unclear. Method of Study Using seminal plasma (SP) from 20 healthy donors, we investigated the impact of human semen on CD4+ T-cell expression of CD127 and CD49d as well as CD4+ CD127low CD49dlow Treg proliferation, apoptosis, and intracellular expression of FoxP3, TGF-beta 1, and IL-10. Results SP reduced CD4+ T-cell expression of CD127 and CD49d and increased the proportion of CD127low CD49dlow Treg. This increase was non-proliferative, mediated in part via the conversion of CD4+ helper T cells into FoxP3- but not FoxP3+ Treg. Additionally, SP induced an increase in intracellular expression of the immunosuppressive cytokine TGF-beta 1 by the FoxP3- but not FoxP3+ Treg. SP had no impact on Treg intracellular expression of IL-10. Conclusion Human seminal plasma fosters the non-proliferative increase in the proportion and immunoregulatory activity of FoxP3- Treg.

引用

页码：322 / 330

页数：9

共 41 条

[1] Expansion of CD4+CD25+ and FOXP3+ regulatory T cells during the follicular phase of the menstrual cycle:: Implications for human reproduction [J].