Site-specific functionalization of RNA molecules by an unnatural base pair transcription system via click chemistry

被引：43

作者：

Ishizuka, Takumi ^{[1
]}

Kimoto, Michiko ^{[1
,2
]}

Sato, Akira ^{[1
]}

Hirao, Ichiro ^{[1
,2
]}

机构：

[1] RIKEN, SSBC, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[2] TagCyx Biotechnol, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 88期

基金：

日本学术振兴会;

关键词：

POSTTRANSCRIPTIONAL CHEMICAL FUNCTIONALIZATION; ENZYMATIC INCORPORATION; GENETIC ALPHABET; TERMINAL ALKYNES; MODIFIED DNA; IN-VIVO; DYNAMICS; RIBOSOME; CYCLOADDITION; TRANSLATION;

D O I：

10.1039/c2cc36293g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Toward new biotechnology by genetic alphabet expansion, we developed an efficient site-specific labeling method for large RNA molecules. The combination of unnatural base pair transcription and post-transcriptional modification by click chemistry enables simple RNA labeling with a wide variety of functional groups at desired positions, in a one-pot reaction.

引用

页码：10835 / 10837

页数：3

共 50 条

[1] Quantitative single cell monitoring of protein synthesis at subcellular resolution using fluorescently labeled tRNA [J].

Barhoom, Sima ;

Kaur, Jaskiran ;

Cooperman, Barry S. ;

Smorodinsky, Nechama I. ;

Smilansky, Zeev ;

Ehrlich, Marcelo ;

Elroy-Stein, Orna .

NUCLEIC ACIDS RESEARCH, 2011, 39 (19)

[2] Fluorescent labeling of tRNAs for dynamics experiments [J].

Betteridge, Thu ;

Liu, Hanqing ;

Gamper, Howard ;

Kirillov, Stanislav ;

Cooperman, Barry S. ;

Hou, Ya-Ming .

RNA, 2007, 13 (09) :1594-1601

[3] tRNA selection and kinetic proofreading in translation [J].

Blanchard, SC ;

Gonzalez, RL ;

Kim, HD ;

Chu, S ;

Puglisi, JD .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2004, 11 (10) :1008-1014

[4] tRNA dynamics on the ribosome during translation [J].

Blanchard, SC ;

Kim, HD ;

Gonzalez, RL ;

Puglisi, JD ;

Chu, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (35) :12893-12898

[5] Folding of the lysine riboswitch: importance of peripheral elements for transcriptional regulation [J].

Blouin, Simon ;

Chinnappan, Raja ;

Lafontaine, Daniel A. .

NUCLEIC ACIDS RESEARCH, 2011, 39 (08) :3373-3387

[6] Directed DNA metallization [J].

Burley, GA ;

Gierlich, J ;

Mofid, MR ;

Nir, H ;

Tal, S ;

Eichen, Y ;

Carell, T .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (05) :1398-1399

[7] Site-specific labeling of the ribosome for single-molecule spectroscopy [J].

Dorywalska, M ;

Blanchard, SC ;

Gonzalez, RL ;

Kim, HD ;

Chu, S ;

Puglisi, JD .

NUCLEIC ACIDS RESEARCH, 2005, 33 (01) :182-189

[8] Click chemistry with DNA [J].

El-Sagheer, Afaf H. ;

Brown, Tom .

CHEMICAL SOCIETY REVIEWS, 2010, 39 (04) :1388-1405

[9] Coupling of ribosomal L1 stalk and tRNA dynamics during translation elongation [J].

Fei, Jingyi ;

Kosuri, Pallav ;

MacDougall, Daniel D. ;

Gonzalez, Ruben L., Jr. .

MOLECULAR CELL, 2008, 30 (03) :348-359

[10]

Fukunaga R, 2008, BIOCHEM BIOPH RES CO, V372, P480, DOI 10.1016/j.bbrc.2008.05.078

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