Raman Spectroscopic Imaging for the Real-Time Detection of Chemical Changes Associated with Docetaxel Exposure

被引:16
作者
Braeutigam, Katharina [1 ,2 ,3 ]
Bocklitz, Thomas [1 ,2 ,3 ]
Schmitt, Michael [1 ,2 ]
Roesch, Petra [1 ,2 ]
Popp, Juergen [1 ,2 ,3 ]
机构
[1] Univ Jena, Inst Phys Chem, D-07743 Jena, Germany
[2] Univ Jena, Abbe Ctr Photon, D-07743 Jena, Germany
[3] Inst Photon Technol, D-07745 Jena, Germany
关键词
anticancer drugs; imaging techniques; living cells; medicinal chemistry; Raman spectroscopy; CANCER-CELL-LINES; MICRO-SPECTROSCOPY; SINGLE CELLS; LIVING CELLS; MICROSPECTROSCOPY; IDENTIFICATION;
D O I
10.1002/cphc.201200800
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
There is an urgent need for methods allowing for a fast, non-invasive, sensitive and selective monitoring of the effectiveness of anticancer drugs during the course of a chemotherapeutic treatment of cancer patients. The possibility of predicting and controlling the efficiency of chemotherapeutic agents for every patient individually enables a personalized therapy with largely improved success rates. The results presented herein demonstrate that Raman microspectroscopy is perfectly suited to monitor the impact of chemotherapeutic agents on living cells. The influence of the clinically well-established chemotherapeutic docetaxel on both the morphology and also biochemistry of living colon cancer cells (HT-29) has been studied by means of Raman spectroscopy in combination with modern chemometric approaches. The work presented paves the way for establishing Raman spectroscopy as a monitoring tool of the effectiveness of a chemotherapy treatment and can therefore be seen as a step towards personalized therapy.
引用
收藏
页码:550 / 553
页数:4
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