Identification of bovine viral diarrhoea virus type 2 in cattle bull semen from southern India and its genetic characterization

被引：3

作者：

Mishra, Niranjan ^{[1
]}

Kalaiyarasu, Semmannan ^{[1
]}

Mallinath, K. C. ^{[2
]}

Rajukumar, Katherukamem ^{[1
]}

Khetan, Rohit K. ^{[1
]}

Gautam, Siddharth ^{[1
]}

Venkatesha, M. D. ^{[2
]}

Byregowda, S. M. ^{[2
]}

机构：

[1] ICAR Res Complex, Natl Inst High Secur Anim Dis, Bhopal 462022, India

[2] Inst Anim Hlth & Biol, Southern Reg Dis Diagnost Lab, Bengaluru 560024, India

来源：

CURRENT SCIENCE | 2018年 / 114卷 / 03期

关键词：

Bovine viral diarrhoea; bull semen; genetic characterization; mandatory testing; TIME RT-PCR; MOLECULAR CHARACTERIZATION; ANTIGENIC CHARACTERIZATION; REPRODUCTIVE CONSEQUENCES; PHYLOGENETIC ANALYSIS; PESTIVIRUSES; VACCINATION; INFECTION; SHEEP;

D O I：

10.18520/cs/v114/i03/666-670

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Although bovine viral diarrhoea virus (BVDV) is prevalent in Indian cattle causing economic losses in cattle farming, its detection in bull semen has not yet been reported. Following passage of raw bull semen (n = 4) on MDBK cells, testing for BVDV was done by antigen ELISA and real-time RT-PCR. BVDV type-2 (BVDV-2) was identified in three samples from southern India by real-time RT-PCR. Genetic typing of the 5 '-UTR sequences classified all the three BVDV strains as BVDV-2a subtype. These were found genetically closely related to the strains from USA, but divergent from the BVDV-2a strains from northern India. Phylogenetic analysis of N-pro sequences confirmed the findings. The results provide evidence of circulation of BVDV-2a strains in southern India. The detection of BVDV in bull semen from India high-lights the importance of mandatory testing of breeding bulls and bull semen for BVDV to minimize the risk of BVDV transmission.

引用

页码：666 / 670

页数：6

共 28 条

[1]

[Anonymous], 2016, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2016, V2, P1

[2] A one-step multiplex real-time RT-PCR for detection and typing of bovine viral diarrhea viruses [J].

Baxi, Mohit ;

McRae, Dorothy ;

Baxi, Shaija ;

Greiser-Wilke, Irene ;

Vilcek, Stefan ;

Amoako, Kinsley ;

Deregt, Dirk .

VETERINARY MICROBIOLOGY, 2006, 116 (1-3) :37-44

[3] Genetic and antigenic characterization of novel pestivirus genotypes:: implications for classification [J].

Becher, P ;

Ramirez, RA ;

Orlich, M ;

Rosales, SC ;

König, M ;

Schweizer, M ;

Stalder, H ;

Schirrmeier, H ;

Thiel, HJ .

VIROLOGY, 2003, 311 (01) :96-104

[4] Phylogenetic analysis of pestiviruses from domestic and wild ruminants [J].

Becher, P ;

Orlich, M ;

Shannon, AD ;

Horner, G ;

Konig, M ;

Thiel, HJ .

JOURNAL OF GENERAL VIROLOGY, 1997, 78 :1357-1366

[5] Genetic and antigenic characterization of bovine viral diarrhoea virus type 2 isolated from cattle in India [J].

Behera, Sthita Pragnya ;

Mishra, Niranjan ;

Vilcek, Stefan ;

Rajukumar, Katherukamem ;

Nema, Ram Kumar ;

Prakash, Anil ;

Kalaiyarasu, S. ;

Dubey, Shiv Chandra .

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2011, 34 (02) :189-196

[6]

Gaede W, 2005, BERL MUNCH TIERARZTL, V118, P113

[7] Noncytopathic bovine viral diarrhea virus can persist in testicular tissue after vaccination of peri-pubertal bulls but prevents subsequent infection [J].

Givens, M. Daniel ;

Riddell, Kay P. ;

Walz, Paul H. ;

Rhoades, Jim ;

Harland, Richard ;

Zhang, YiJing ;

Galik, Patricia K. ;

Brodersen, Bruce W. ;

Cochran, Anna M. ;

Brock, Kenny V. ;

Carson, Robert L. ;

Stringfellow, David A. .

VACCINE, 2007, 25 (05) :867-876

[8] Detection of bovine viral diarrhea virus in semen obtained after inoculation of seronegative postpubertal bulls [J].

Givens, MD ;

Heath, AM ;

Brock, KV ;

Brodersen, BW ;

Carson, RL ;

Stringfellow, DA .

AMERICAN JOURNAL OF VETERINARY RESEARCH, 2003, 64 (04) :428-434

[9] Clinical and reproductive consequences of using BVDV-contaminated semen in artificial insemination in a beef herd in Argentina [J].

Gonzalez Altamiranda, E. A. ;

Kaiser, G. G. ;

Weber, N. ;

Leunda, M. R. ;

Pecora, A. ;

Malacari, D. A. ;

Moran, Oscar ;

Campero, C. M. ;

Odeon, A. C. .

ANIMAL REPRODUCTION SCIENCE, 2012, 133 (3-4) :146-152

[10] Reproductive consequences of infection with bovine viral diarrhea virus [J].

Grooms, DL .

VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE, 2004, 20 (01) :5-+

← 1 2 3 →