Synthesis of polyhydroxyalkanoate in the peroxisome of Pichia pastoris

被引：21

作者：

Poirier, Y ^{[1
]}

Erard, N ^{[1
]}

Petétot, JMC ^{[1
]}

机构：

[1] Univ Lausanne, Inst Ecol, Lab Biotechnol Vegetale, CH-1015 Lausanne, Switzerland

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 207卷 / 01期

关键词：

beta-oxidation; peroxisome; polyhydroxyalkanoate; Pichia pastoris;

D O I：

10.1111/j.1574-6968.2002.tb11035.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Polyhydroxyalkanoates (PHAs) are polyesters naturally produced by bacteria that have properties of biodegradable plastics and elastomers. A PHA synthase from Pseudomonas aeruginosa modified at the carboxy-end for peroxisomal targeting was transformed in Pichia pastoris, The PHA synthase was expressed under the control of the promoter of the P. pastoris acyl-CoA oxidase gene, Synthesis of up to 1% medium-chain-length PHA per g dry weight was dependent on both the expression of the PHA synthase and the presence of oleic acid in the medium. PHA accumulated as inclusions within the peroxisomes. P. pastoris could be used as a model system to study how peroxisomal metabolism needs to be modified to increase PHA production in other eukaryotes, such as plants. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：97 / 102

页数：6

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