Determination of nucleotide sequence of SRY gene in sika deer (Cervus nippon)

被引：3

作者：

Fukui, E ^{[1
]}

Koganezawa, M ^{[1
]}

Yoshizawa, M ^{[1
]}

机构：

[1] Utsunomiya Univ, Fac Agr, Utsunomiya, Tochigi 3218505, Japan

来源：

ANIMAL SCIENCE JOURNAL | 2006年 / 77卷 / 02期

关键词：

nucleotide sequence; sika deer; SRY gene;

D O I：

10.1111/j.1740-0929.2006.00345.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aim of the present study was to determine the whole nucleotide sequence of the open reading frame of the sex-determining region Y (SRY-ORF) in wild sika deer. The SRY gene of wild sika deer was obtained by polymerase chain reaction (PCR) with DNA from blood samples. The whole nucleotide sequence of the SRY-ORF in Wild sika deer consisted of 687 bp and encoded 229 deduced amino acids. In comparison with the bovine SRY gene, the percentage of nucleotide sequence homology was 91.0% in the overall ORF, and those of the N-terminal, high mobility group (HMG) box, and C-terminal regions within ORF were 88.9%, 96.2% and 87.9%, respectively. The nucleotide sequences of sika deer SRY-ORF characterized in the present study can be used for phylogenetic analysis or sexing in wild sika deer.

引用

页码：250 / 252

页数：3

共 9 条

[1] BOVINE SRY GENE LOCUS - CLONING AND TESTICULAR EXPRESSION [J].

DANEAU, I ;

HOUDE, A ;

ETHIER, JF ;

LUSSIER, JG ;

SILVERSIDES, DW .

BIOLOGY OF REPRODUCTION, 1995, 52 (03) :591-599

[2]

EMMANUEL JP, 1993, NUCLEIC ACIDS RES, V21, P2772

[3] EVOLUTION OF SEX DETERMINATION AND THE Y-CHROMOSOME - SRY-RELATED SEQUENCES IN MARSUPIALS [J].

FOSTER, JW ;

BRENNAN, FE ;

HAMPIKIAN, GK ;

GOODFELLOW, PN ;

SINCLAIR, AH ;

LOVELLBADGE, R ;

SELWOOD, L ;

RENFREE, MB ;

COOPER, DW ;

GRAVES, JAM .

NATURE, 1992, 359 (6395) :531-533

[4] A GENE-MAPPING TO THE SEX-DETERMINING REGION OF THE MOUSE Y-CHROMOSOME IS A MEMBER OF A NOVEL FAMILY OF EMBRYONICALLY EXPRESSED GENES [J].

GUBBAY, J ;

COLLIGNON, J ;

KOOPMAN, P ;

CAPEL, B ;

ECONOMOU, A ;

MUNSTERBERG, A ;

VIVIAN, N ;

GOODFELLOW, P ;

LOVELLBADGE, R .

NATURE, 1990, 346 (6281) :245-250

[5]

Kageyama Soichi, 1992, Animal Science and Technology, V63, P1059

[6] A novel Sry-downstream cellular event which preserves the readily available energy source of glycogen in mouse sex differentiation [J].

Matoba, S ;

Kanai, Y ;

Kidokoro, T ;

Kanai-Azuma, M ;

Kawakami, H ;

Hayashi, Y ;

Kurohmaru, M .

JOURNAL OF CELL SCIENCE, 2005, 118 (07) :1449-1459

[7] A GENE FROM THE HUMAN SEX-DETERMINING REGION ENCODES A PROTEIN WITH HOMOLOGY TO A CONSERVED DNA-BINDING MOTIF [J].

SINCLAIR, AH ;

BERTA, P ;

PALMER, MS ;

HAWKINS, JR ;

GRIFFITHS, BL ;

SMITH, MJ ;

FOSTER, JW ;

FRISCHAUF, AM ;

LOVELLBADGE, R ;

GOODFELLOW, PN .

NATURE, 1990, 346 (6281) :240-244

[8] RAPID-SEQUENCE EVOLUTION OF THE MAMMALIAN SEX-DETERMINING GENE SRY [J].

WHITFIELD, LS ;

LOVELLBADGE, R ;

GOODFELLOW, PN .

NATURE, 1993, 364 (6439) :713-715

[9]

Wilson PJ, 1998, J FORENSIC SCI, V43, P477

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