A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins

被引：0

作者：

Lukinavicius, Grazvydas ^{[1
]}

Umezawa, Keitaro ^{[1
]}

Olivier, Nicolas ^{[2
]}

Honigmann, Alf ^{[3
]}

Yang, Guoying ^{[4
]}

Plass, Tilman ^{[5
]}

Mueller, Veronika ^{[3
]}

Reymond, Luc ^{[1
]}

Correa, Ivan R., Jr. ^{[6
]}

Luo, Zhen-Ge ^{[7
,8
]}

Schultz, Carsten ^{[5
]}

Lemke, Edward A. ^{[5
]}

Heppenstall, Paul ^{[4
]}

Eggeling, Christian ^{[3
,9
]}

Manley, Suliana ^{[2
]}

Johnsson, Kai ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Chem Sci & Engn ISIC, Inst Bioengn, Natl Ctr Competence Res NCCR Chem Biol, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Lab Expt Biophys, NCCR Chem Biol, CH-1015 Lausanne, Switzerland

[3] Max Planck Inst Biophys Chem, Dept NanoBiophoton, D-37077 Gottingen, Germany

[4] European Mol Biol Lab, Mouse Biol Unit, I-00015 Monterotondo, RM, Italy

[5] European Mol Biol Lab, D-69117 Heidelberg, Germany

[6] New England Biolabs Inc, Ipswich, MA 01938 USA

[7] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, Shanghai 200031, Peoples R China

[8] Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Neurosci, Shanghai 200031, Peoples R China

[9] Univ Oxford, Weatherall Inst Mol Med, Med Res Council Human Immunol Unit, Oxford OX1 2JD, England

来源：

NATURE CHEMISTRY | 2013年 / 5卷 / 02期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

DIELS-ALDER REACTIONS; LIVING CELLS; FUSION PROTEINS; FAR-RED; FLUORESCENCE MICROSCOPY; SYNTHETIC FLUOROPHORES; STIMULATED-EMISSION; STED NANOSCOPY; SI-RHODAMINE; MOUSE-BRAIN;

D O I：

10.1038/NCHEM.1546

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can be implemented flexibly in living cells and in vivo. However, the design of synthetic fluorophores that combine all of these properties has proved to be extremely difficult. Here, we introduce a biocompatible near-infrared silicon-rhodamine probe that can be coupled specifically to proteins using different labelling techniques. Importantly, its high permeability and fluorogenic character permit the imaging of proteins in living cells and tissues, and its brightness and photostability make it ideally suited for live-cell super-resolution microscopy. The excellent spectroscopic properties of the probe combined with its ease of use in live-cell applications make it a powerful new tool for bioimaging.

引用

页码：132 / 139

页数：8