Highly Elastic Micropatterned Hydrogel for Engineering Functional Cardiac Tissue

被引:199
作者
Annabi, Nasim [1 ,2 ,3 ]
Tsang, Kelly [1 ,2 ,4 ]
Mithieux, Suzanne M. [5 ]
Nikkhah, Mehdi [1 ,2 ]
Ameri, Afshin [1 ,2 ]
Khademhosseini, Ali [1 ,2 ,3 ]
Weiss, Anthony S. [5 ,6 ,7 ]
机构
[1] Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Med,Ctr Biomed Engn, Boston, MA 02139 USA
[2] MIT, Harvard MIT Div Hlth Sci & Technol, Cambridge, MA 02139 USA
[3] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA
[4] Monash Univ, Dept Mat Engn, Melbourne, Vic 3004, Australia
[5] Univ Sydney, Sch Mol Biosci, Sydney, NSW 2006, Australia
[6] Univ Sydney, Charles Perkins Ctr, Sydney, NSW 2006, Australia
[7] Univ Sydney, Bosch Inst, Sydney, NSW 2006, Australia
基金
美国国家科学基金会; 澳大利亚研究理事会; 英国医学研究理事会; 美国国家卫生研究院;
关键词
tropoelastin; microfabrication; cardiomyocyte; cell alignment; elasticity; IN-VITRO MODEL; ELECTRICAL-STIMULATION; CARDIOMYOCYTE SHEETS; MUSCLE; BIOREACTOR; ALIGNMENT; CULTURES; LAMININ; SYSTEMS; FILMS;
D O I
10.1002/adfm.201300570
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Heart failure is a major international health issue. Myocardial mass loss and lack of contractility are precursors to heart failure. Surgical demand for effective myocardial repair is tempered by a paucity of appropriate biological materials. These materials should conveniently replicate natural human tissue components, convey persistent elasticity, promote cell attachment, growth and conformability to direct cell orientation and functional performance. Here, microfabrication techniques are applied to recombinant human tropoelastin, the resilience-imparting protein found in all elastic human tissues, to generate photocrosslinked biological materials containing well-defined micropatterns. These highly elastic substrates are then used to engineer biomimetic cardiac tissue constructs. The micropatterned hydrogels, produced through photocrosslinking of methacrylated tropoelastin (MeTro), promote the attachment, spreading, alignment, function, and intercellular communication of cardiomyocytes by providing an elastic mechanical support that mimics their dynamic mechanical properties in vivo. The fabricated MeTro hydrogels also support the synchronous beating of cardiomyocytes in response to electrical field stimulation. These novel engineered micropatterned elastic gels are designed to be amenable to 3D modular assembly and establish a versatile, adaptable foundation for the modeling and regeneration of functional cardiac tissue with potential for application to other elastic tissues.
引用
收藏
页码:4950 / 4959
页数:10
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