Assay of the deuterium enrichment of water via acetylene

被引：0
作者：
Previs, SF
Hazey, JW
Diraison, F
Beylot, M
David, F
Brunengraber, H
机构：
[1] CASE WESTERN RESERVE UNIV,DEPT NUTR,CLEVELAND,OH 44106
[2] CASE WESTERN RESERVE UNIV HOSP,DEPT SURG,INTEGRATED SURG PROGRAM,CLEVELAND,OH 44106
[3] INSERM U197,F-69008 LYON,FRANCE
来源：
JOURNAL OF MASS SPECTROMETRY | 1996年 / 31卷 / 06期
关键词：
deuterium oxide; calcium carbide; acetylene; lipogenesis; gluconeogenesis; gas chromatography mass spectrometry;
D O I：
10.1002/(SICI)1096-9888(199606)31:6<639::AID-JMS336>3.0.CO;2-S
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A technique is presented for measuring the H-2 enrichment of water in biological samples when this enrichment is greater than 0.2%. The sample is reacted with calcium carbide to form acetylene gas, which is determined by gas chromatography electron impact ionization mass spectrometry. Ion-molecule reactions, resulting in proton abstraction, are minimized by lowering the electron ionization energy from the usual 70 eV to 45 eV, This technique is much more rapid and economical than the classical isotope ratio mass spectrometric assay of the enrichment of hydrogen gas derived from reduction of water.
引用
收藏
页码：639 / 642
页数：4
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