H2O2 mediates O2 toxicity in cultured fetal rat distal lung epithelial cells

It is unknown which of the reactive oxygen species is primarily responsible for the cytotoxicity of 95% O-2 for rat distal fetal lung epithelial cells in vitro. Incubation of cells with 25 U/ml polyethylene glycol (PEG)-conjugated SOD and 50 U/ml PEG-catalase, but not PEG-SOD or SOD mimics alone, significantly reduced 95% O-2-mediated cytotoxicity. Liposome-entrapped catalase, without SOD, also significantly reduced 95% O-2-mediated cytotoxicity. Increased formation of lipid hydroperoxides, as assessed by the formation of 8-isoprostane and aldehydes, was attenuated by both 100 mu M Trolox, a vitamin E analogue, and by 5 mu M U74389G, an amino steroid. Trolox. but not U74389G, prevented an increase in cell-derived H2O2, hydroxyl radical and 95% O-2-mediated cytotoxicity, An increase in hydroxyl radical formation, but not cell death, observed in 95% O-2, was prevented by 0.1 mu M phenanthrolene, a cell permeant iron chelator. DNA extracts of rat distal fetal lung epithelial cells maintained under serum-free conditions had an electrophoretic pattern consistent with some degree of apoptosis. However, no increase in laddering was seen with exposure to 95% O-2, These data are consistent with hydrogen peroxide, but not lipid hydroperoxides or hydroxyl radical, being a critical effector of O-2-mediated necrotic cell death in distal lung epithelial cells. (C) 1999 Elsevier Science Inc.