Fast macro-scale transmission imaging of microvascular networks using KESM

被引：28

作者：

Mayerich, David ^{[1
]}

Kwon, Jaerock ^{[2
]}

Sung, Chul ^{[4
]}

Abbott, Louise ^{[3
]}

Keyser, John ^{[4
]}

Choe, Yoonsuck ^{[4
]}

机构：

[1] Univ Illinois, Beckman Inst Adv Sci & Technol, Urbana, IL 61801 USA

[2] Kettering Univ, Dept Elect & Comp Engn, Flint, MI 48504 USA

[3] Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA

[4] Texas A&M Univ, Dept Comp Sci & Engn, College Stn, TX 77843 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2011年 / 2卷 / 10期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

HUMAN CEREBRAL-CORTEX; MOUSE-BRAIN; TOMOGRAPHY; PERFUSION;

D O I：

10.1364/BOE.2.002888

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Accurate microvascular morphometric information has significant implications in several fields, including the quantification of angiogenesis in cancer research, understanding the immune response for neural prosthetics, and predicting the nature of blood flow as it relates to stroke. We report imaging of the whole mouse brain microvascular system at resolutions sufficient to perform accurate morphometry. Imaging was performed using Knife-Edge Scanning Microscopy (KESM) and is the first example of this technique that can be directly applied to clinical research. We are able to achieve approximate to 0.7 mu m resolution laterally with 1 mu m depth resolution using serial sectioning. No alignment was necessary and contrast was sufficient to allow segmentation and measurement of vessels. (C) 2011 Optical Society of America

引用

页码：2888 / 2896

页数：9

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