The novel organization and complete sequence of the ribosomal RNA gene of Nosema bombycis

被引：68

作者：

Huang, WF

Tsai, SJ

Lo, CF

Soichi, Y

Wang, CH ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Entomol, Taipei 106, Taiwan

[2] Natl Taiwan Univ, Dept Zool, Taipei 106, Taiwan

[3] Ibaraki Univ, Fac Educ, Biol Lab, Mito, Ibaraki 3108512, Japan

来源：

FUNGAL GENETICS AND BIOLOGY | 2004年 / 41卷 / 05期

关键词：

Nosema; rRNA organization; microsporidia;

D O I：

10.1016/j.fgb.2003.12.005

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We present here for the first time the complete DNA sequence data (4301 bp) of the ribosomal RNA (rRNA) gene of the microsporidian type species, Nosema bombycis. Sequences for the large subunit gene (LSUrRNA: 2497 bp, GenBank Accession No. AY211393), the internal transcribed spacer (ITS: 179 bp, GenBank Accession No. AY211394), the small subunit gene (SSUrRNA: 1232 bp), intergenic spacer (IGS: 279 bp), and 5S region (114 bp) are also given, and the secondary structure of the large subunit is discussed. The organization of the N. bombycis rRNA gene is LSUrRNA-ITS-SSUrRNA-IGS-5S. This novel arrangement, in which the LSU is 5' of the SSU, is the reverse of the organizational sequence (i.e., SSU-ITS-LSU) found in all previously reported microsporidian rRNAs, including Nosema apis. This unique character in the type species may have taxonomic implications for the members of the genus Nosema. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：473 / 481

页数：9

共 37 条

[1] PHYLOGENETIC-RELATIONSHIPS AMONG VAIRIMORPHA AND NOSEMA SPECIES (MICROSPORA) BASED ON RIBOSOMAL-RNA SEQUENCE DATA
BAKER, MD
VOSSBRINCK, CR
MADDOX, JV
UNDEEN, AH
[J]. JOURNAL OF INVERTEBRATE PATHOLOGY, 1994, 64 (02) : 100 - 106
[2] SMALL-SUBUNIT RIBOSOMAL DNA PHYLOGENY OF VARIOUS MICROSPORIDIA WITH EMPHASIS ON AIDS-RELATED FORMS
BAKER, MD
VOSSBRINCK, CR
DIDIER, ES
MADDOX, JV
SHADDUCK, JA
[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1995, 42 (05) : 564 - 570
[3] Becnel James J., 1999, P447
[4] The secondary structure of Nosema apis large subunit ribosomal RNA
De Rijk, P
Gatehouse, HS
De Wachter, R
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1998, 1442 (2-3): : 326 - 328
[5] RnaViz 2: an improved representation of RNA secondary structure
De Rijk, P
Wuyts, J
De Wachter, R
[J]. BIOINFORMATICS, 2003, 19 (02) : 299 - 300
[6] Database on the structure of large ribosomal subunit RNA
De Rijk, P
Caers, A
Van de Peer, Y
De Wachter, R
[J]. NUCLEIC ACIDS RESEARCH, 1998, 26 (01) : 183 - 186
[7] RnaViz, a program for the visualisation of RNA secondary structure
DeRijk, P
DeWachter, R
[J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (22) : 4679 - 4684
[8] DERIJK P, 1993, COMPUT APPL BIOSCI, V9, P735
[9] INFERRING PHYLOGENIES FROM DNA-SEQUENCES OF UNEQUAL BASE COMPOSITIONS
GALTIER, N
GOUY, M
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) : 11317 - 11321
[10] The ribosomal RNA gene region of Nosema apis (microspora): DNA sequence for small and large subunit rRNA genes and evidence of a large tandem repeat unit size
Gatehouse, HS
Malone, LA
[J]. JOURNAL OF INVERTEBRATE PATHOLOGY, 1998, 71 (02) : 97 - 105

← 1 2 3 4 →