Iridoid and aromatic glycosides from Scrophularia ningpoensis HEMSL. and their inhibition of [Ca2+]i increase induced by KCl

被引:39
作者
Chen, Bin [1 ]
Liu, Yan [2 ]
Liu, Hong-Wei [3 ]
Wang, Nai-Li [1 ,4 ]
Yang, Bao-Feng [2 ]
Yao, Xin-Sheng [1 ]
机构
[1] Shenyang Pharmaceut Univ, Dept Nat Prod Chem 81, Shenyang 110016, Peoples R China
[2] Harbin Med Univ, Dept Pharmacol, Harbin 150081, Peoples R China
[3] Capital Univ Med Sci, Sch Chem Biol & Pharmaceut Sci, Beijing 100069, Peoples R China
[4] Tradit Chinese Med & Nat Prod Shenzhen, Key Lab Res & Dev New Drugs, Shenzhen 518057, Peoples R China
关键词
D O I
10.1002/cbdv.200890161
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Bioassay-guided fractionation of EtOH extract of the roots of Scrophularia ningpoensis HEMSL. resulted in the isolation of three new iridoid glycosides, i.e. 6 ''-O-caffeoylharpagide (1), 6 ''-O-feruloylharpagide (2), and 6 ''-O-beta-glucopyranosylharpagoside (3), and five new aromatic glycosides, i.e., 2-(3-hydroxy-4-methoxyphenyl)ethyl O-alpha-arabinopyranosyl-(1 -> 6)-O-alpha-rhamnopyranosyl-(1 -> 3)-O-beta-glucopyranoside (4), phenyl O-beta-xylopyranosyl-(1 -> 6)-O-beta-glucopyranoside (5), 3-methylphenyl O-beta-xylopyranosyl-(1 -> 6)-O-beta-glucopyranoside (6), 6-O-cinnamoyl beta-fructofuranosyl-(2 -> 1)-O-alpha-glucopyranosyl-(6 -> 1)-O-alpha-glucopyranoside (7), and 6-O-feryloyl beta-fructofuranosyl-(2 -> 1)-O-alpha-glucopyranosyl-(6 -> 1)-O-alpha-glucopyranoside (8), together with four known compounds, i.e., 6 ''-O-alpha-D-galactopyranosyl harpagoside (9), 6 ''-O-(p-coumaroyl) harpagide (10), harpagoside (11), and angoroside C (12). Activity of the isolated compounds on [Ca2+](i) increase induced by KCl was evaluated on rat cardiac myocytes using confocal laser scanning microscopy. Iridoid glycosides 1, 10, and 11, and aromatic glycosides 5 and 6 significantly inhibited the increase of [Ca2-](i) induced by KCl at 100 mu M.
引用
收藏
页码:1723 / 1735
页数:13
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