microRNAs associated with the different human Argonaute proteins

被引:155
作者
Dueck, Anne [1 ]
Ziegler, Christian [1 ]
Eichner, Alexander [2 ]
Berezikov, Eugene [3 ,4 ]
Meister, Gunter [1 ]
机构
[1] Univ Regensburg, Biochem Ctr Regensburg BZR, Lab RNA Biol, D-93053 Regensburg, Germany
[2] IST Austria, A-3400 Klosterneuburg, Austria
[3] Univ Med Ctr Utrecht, Hubrecht Inst Dev Biol & Stem Cell Res, Royal Netherlands Acad Arts & Sci, NL-3584 CT Utrecht, Netherlands
[4] Univ Groningen, Univ Med Ctr Groningen, European Res Inst Biol Ageing, NL-9713 AV Groningen, Netherlands
基金
欧洲研究理事会;
关键词
MESSENGER-RNA TRANSLATION; PASSENGER-STRAND; SIRNA; IDENTIFICATION; BIOGENESIS; MECHANISMS; DROSOPHILA; COMPLEXES; CLEAVAGE; CELLS;
D O I
10.1093/nar/gks705
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
MicroRNAs (miRNAs) are small noncoding RNAs that function in literally all cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them to specific target sites located in the 3'-untranslated region (3'-UTR) of target mRNAs leading to translational repression and deadenylation-induced mRNA degradation. Most miRNAs are processed from hairpin-structured precursors by the consecutive action of the RNase III enzymes Drosha and Dicer. However, processing of miR-451 is Dicer independent and cleavage is mediated by the endonuclease Ago2. Here we have characterized miR-451 sequence and structure requirements for processing as well as sorting of miRNAs into different Ago proteins. Pre-miR-451 appears to be optimized for Ago2 cleavage and changes result in reduced processing. In addition, we show that the mature miR-451 only associates with Ago2 suggesting that mature miRNAs are not exchanged between different members of the Ago protein family. Based on cloning and deep sequencing of endogenous miRNAs associated with Ago1-3, we do not find evidence for miRNA sorting in human cells. However, Ago identity appears to influence the length of some miRNAs, while others remain unaffected.
引用
收藏
页码:9850 / 9862
页数:13
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