Evaluation of new broth media for microdilution antibiotic susceptibility testing of Lactobacilli, Pediococci, Lactococci, and Bifidobacteria

被引:211
作者
Klare, I
Konstabel, C
Müller-Bertling, S
Reissbrodtl, R
Huys, G
Vancanneyt, M
Swings, J
Goossens, H
Witte, W
机构
[1] Robert Koch Inst, Wernigerode Branch, D-38855 Wernigerode, Germany
[2] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium
[3] Univ Ghent, BCCM LMG Bacteria Collect, B-9000 Ghent, Belgium
[4] Univ Instelling Antwerp, B-2610 Antwerp, Belgium
关键词
D O I
10.1128/AEM.71.12.8982-8986.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Nine pure or mixed broth media were evaluated for their suitabilities to determine MICs in a microdilution test of 19 antibacterial agents for lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Lactococcus, and Bifidobacterium. A mixed formulation of Iso-Sensitest broth (90%) and deMan-Rogosa-Sharpe broth (10%) with or without supplementation with L-cysteine, referred to as the LAB susceptibility test medium, provided the most optimal medium basis in terms of growth support of nonenterococcal LAB and correct indication of MICs of international control strains.
引用
收藏
页码:8982 / 8986
页数:5
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