Improved Multiplex PCR Strategy for Rapid Assignment of the Four Major Escherichia coli Phylogenetic Groups

被引：151

作者：

Doumith, M. ^{[1
]}

Day, M. J. ^{[1
]}

Hope, R. ^{[2
]}

Wain, J. ^{[1
,3
]}

Woodford, N. ^{[1
]}

机构：

[1] Hlth Protect Agcy, Microbiol Serv Colindale, London, England

[2] Hlth Protect Agcy, Hlth Protect Serv Colindale, London, England

[3] Univ E Anglia, Norwich Med Sch, Norwich NR4 7TJ, Norfolk, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 09期

关键词：

LACTAMASES; STRAINS;

D O I：

10.1128/JCM.01468-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Using data from whole-genome projects, an updated multiplex PCR strategy was developed to assign Escherichia coli isolates rapidly to major phylogenetic groups. This assay accommodates sequence variations detected within target sequences, thereby increasing sensitivity and reliability. It was validated using 185 isolates of known sequence types and showed improved congruence with multilocus sequence typing data.

引用

页码：3108 / 3110

页数：3

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