CUB-domain containing protein 1 represses the epithelial phenotype of pancreatic cancer cells

被引：21
作者：
Miura, Shin [1 ]
Hamada, Shin [1 ]
Masamune, Atsushi [1 ]
Satoh, Kennichi [2 ]
Shimosegawa, Tooru [1 ]
机构：
[1] Tohoku Univ, Div Gastroenterol, Grad Sch Med, Aoba Ku, Sendai, Miyagi 9808574, Japan
[2] Miyagi Canc Ctr Res Inst, Div Canc Stem Cell, Natori, Miyagi 9811293, Japan
来源：
EXPERIMENTAL CELL RESEARCH | 2014年 / 321卷 / 02期
基金：
日本学术振兴会;
关键词：
CUB-domain containing protein 1; Bone morphogenetic protein 4; Transforming growth factor-beta; Epithelial-mesenchymal transition; Extracellular signal-regulated kinase; MESENCHYMAL TRANSITION; COLORECTAL-CANCER; STELLATE CELLS; TYROSINE PHOSPHORYLATION; PERITONEAL DISSEMINATION; SIGNAL-TRANSDUCTION; TUMOR-CELLS; STEM-CELLS; CDCP1; IDENTIFICATION;
D O I：
10.1016/j.yexcr.2013.12.019
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The prognosis of pancreatic cancer is dismal due to the frequent metastasis and invasion to surrounding organs. Numerous molecules are involved in the malignant behavior of pancreatic cancer cells, but the entire process remains unclear. Several reports have suggested that CUBdomain containing protein-1 (CDCP1) is highly expressed in pancreatic cancer, but its impact on the invasive growth and the upstream regulator remain elusive. To clarify the role of CDCP1 in pancreatic cancer, we here examined the effects of CDCP1 knockdown on the cell behaviors of pancreatic cancer cells. Knockdown of CDCP1 expression in Panc-1 resulted in reduced cellular migration accompanied by the increased expression of E-cadherin and decreased expression of N-cadherin. Knockdown of CDCP1 attenuated the spheroid formation and resistance against gemcitabine, which are some of the cancer stem cell-related phenotypes. Bone morphogenetic protein 4 (BMP4) was found to induce CDCP1 expression via the extracellular signal regulated kinase pathway, suggesting that CDCP1 has a substantial role in the BMP4-induced epithelialmesenchymal transition. These results indicate that CDCP1 represses the epithelial phenotype of pancreatic cancer cells. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码：209 / 218
页数：10
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