The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green

被引：38

作者：

Zhao, Jinfu ^{[1
]}

Bertoglio, Bryan A. ^{[1
,2
]}

Devinney, Michael J., Jr. ^{[3
]}

Dineley, Kirk E. ^{[4
]}

Kay, Alan R. ^{[1
]}

机构：

[1] Univ Iowa, Dept Biol, Iowa City, IA 52242 USA

[2] Univ Iowa, Dept Neurosurg, Iowa City, IA 52242 USA

[3] Univ Pittsburgh, Dept Pharmacol, Pittsburgh, PA 15261 USA

[4] Francis Marion Univ, Dept Biol, Florence, SC 29501 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 384卷 / 01期

关键词：

Fluorescence; Zinc; Copper; Nickel; Manganese; Iron; Cobalt; Cadmium; FLUORESCENT DETECTION; NEURONAL APOPTOSIS; COPPER; CHEMISTRY; DESIGN; INDICATORS; SENSOR; CELLS; IRON;

D O I：

10.1016/j.ab.2008.09.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Zinc-sensitive fluorescent probes have become increasingly important in the investigation of the cellular roles of zinc. There is, however, little information on how the other transition metals in cells may influence the measurement of zinc. We have characterized in vitro the interaction of the nominal zinc indicators FluoZin-3 and Newport Green with all the cationic transition metals found within cells, Cr, Mn, Fe, Co, and Cu, as well as Ni and Cd, by measuring their dissociation constants. In addition, we have shown how FluoZin-3 can be used to quantify the concentration of copper in a cell-free assay and report that the fluorescence of Newport Green is boosted by both Cu(I) and Fe(II). Furthermore, we have introduced diagnostics for detecting the interference of metals other than zinc with its measurement within cells. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：34 / 41

页数：8

共 38 条

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