An in vitro study on the photosensitivity of 5-aminolevulinic acid conjugated gold nanoparticles

被引：34
作者：
Mohammadi, Zahra [1 ]
Sazgarnia, Ameneh [2 ]
Rajabi, Omid [3 ]
Soudmand, Samaneh [4 ]
Esmaily, Habibollah [5 ]
Sadeghi, Hamid Reza [4 ]
机构：
[1] Bobol Univ Med Sci, Dept & Res Ctr Med Phys, Babol Sar, Iran
[2] Mashhad Univ Med Sci, Med Phys Res Ctr, Mashhad, Iran
[3] Mashhad Univ Med Sci, Sch Pharm, Dept Med Chem, Mashhad, Iran
[4] Med Univ Mashhad, Med Phys Res Ctr, Mashhad, Iran
[5] Med Univ Mashhad, Dept Biostat, Mashhad, Iran
来源：
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY | 2013年 / 10卷 / 04期
关键词：
5-Aminolevulinic acid; Gold nanoparticle (GNP); Photodynamic therapy (PDT); Protoporphyrin IX (PpIX); Mel-Rm cell line; MEDIATED PHOTODYNAMIC THERAPY; CANCER; RADIATION; EFFICACY; MICE;
D O I：
10.1016/j.pdpdt.2013.03.010
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background: In this study, the photosensitivity of 5-aminolevulinic acid (5-ALA) conjugated gold nanoparticles (conjugate) has been investigated on the MeL-Rm cell line. Methods: After culture and proliferation of the Mel-Rm cell line, cell toxicity and the suitable cell incubation time of the conjugate were determined. Photosensitivity of the conjugate and free 5-ALA was then studied in optical doses of 20, 40, 60 and 80J/cm(2) while tight irradiation was applied by a He-Ne laser. Results: A 4-h time interval was determined as the optimum incubation time for 5-ALA and the conjugate. PpIX induction into the cells showed a significant increase after incubation with the conjugate in comparison to 5-ALA alone. Also, the conjugate resulted in a two times higher cell death rate compared to free 5-ALA. Conclusion: The conjugate can be used as an appropriate delivery agent for 5-ALA and provide efficient cell destruction during PDT. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：382 / 388
页数：7
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