Identification of Spoilage fungus from strawberry fruit by PCR

Strawberry fruit easily suffers from fruit disease caused by fungus infection because of its great susceptibility to microbial spoilage. In present paper the fungus pathogen was isolated from infected strawberry fruits. The molecular method was used to identify the species of the fungus. The internal transcribed spacer (ITS) region was amplified by PCR with the pair of ITS universal primers after extracting the genomic DNA from the isolate. The sequence alignment results indicated that the identity was 95% between the strain F1 and the fungus(GenBank: FN667943). Then the phylogenetic tree was constructed based on the sequence of ITS region of the fungal rRNA operon. According to the sequences of ribosomal DNA-ITS and phylogenetic analysis, the fungi was preliminaryly identified as Trichocomaceae. So the pathogen isolated from the infected strawberry fruits in Tianyi strawberry ecology located in Changping district, Beijing, China is a species of Trichocomaceae.