Improvement and modeling of culture parameters to enhance biomass and lipid production by the oleaginous yeast Cryptococcus curvatus grown on acetate

被引：59
作者：
Beligon, Vanessa [1 ]
Poughon, Laurent [1 ,2 ]
Christophe, Gwendoline [1 ,2 ]
Lebert, Andre [1 ,2 ]
Larroche, Christian [1 ,2 ]
Fontanille, Pierre [1 ,2 ]
机构：
[1] Univ Clermont Ferrand, Inst Pascal, UMR 6602, CNRS,TSA 60026,CS 60026, F-63178 Aubiere, France
[2] Univ Clermont Ferrand, LABEX IMobS3, TSA 60026, CS 60026, F-63178 Aubiere, France
来源：
BIORESOURCE TECHNOLOGY | 2015年 / 192卷
关键词：
Volatile fatty acids; Cryptococcus curvatus; Microbial lipids; Bioconversion; Biodiesel; VOLATILE FATTY-ACIDS; RHODOSPORIDIUM-TORULOIDES Y4; CELL-DENSITY CULTIVATION; YARROWIA-LIPOLYTICA; BIODIESEL PRODUCTION; LIPOMYCES-STARKEYI; MICROBIAL LIPIDS; CARBON-SOURCES; ACCUMULATION; GLYCEROL;
D O I：
10.1016/j.biortech.2015.06.041
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
The improvement of culture parameters for lipid production from acetate as carbon source was investigated using the oleaginous yeast Cryptococcus curvatus. A new pH regulation system dispensing acetate was developed for fed-batch culture and allowed obtaining nearly 80 g/L biomass within 60 h with a maximal growth rate of 0.28 h(-1). A biological model was developed from experimental data. The influence of three C/N ratios of 300, 500 and 900 were tested during a multi-phases process on lipid accumulation. The C/N ratio of 300 was reported to be the most suitable for lipid storage. No significant increase of lipids content was obtained with higher value. A maximal content of 60% DCW of lipid was obtained. The determination of fatty acids profiles of the microbial oils has confirmed that the valorization of acetate by microbial oils production was a promising perspective. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：582 / 591
页数：10
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