The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells

被引：161

作者：

Kanaya, Takashi ^{[2
,3
]}

Hase, Koji ^{[2
,3
]}

Takahashi, Daisuke ^{[3
]}

Fukuda, Shinji ^{[2
,3
]}

Hoshino, Katsuaki ^{[1
,4
]}

Sasaki, Izumi ^{[1
,4
,5
]}

Hemmi, Hiroaki ^{[1
,4
]}

Knoop, Kathryn A. ^{[6
]}

Kumar, Nachiket ^{[6
]}

Sato, Mayuko ^{[7
]}

Katsuno, Tatsuro ^{[8
]}

Yokosuka, Osamu ^{[8
]}

Toyooka, Kiminori ^{[7
]}

Nakai, Kumiko ^{[3
]}

Sakamoto, Ayako ^{[3
]}

Kitahara, Yuuki ^{[3
,9
]}

Jinnohara, Toshi ^{[2
,3
]}

McSorley, Stephen J. ^{[10
]}

Kaisho, Tsuneyasu ^{[1
,4
,5
]}

Williams, Ifor R. ^{[6
]}

Ohno, Hiroshi ^{[2
,3
,9
]}

机构：

[1] RIKEN, Res Ctr Allergy & Immunol, Host Def Lab, Kanagawa, Japan

[2] Yokohama City Univ, Grad Sch Nanobiosci, Yokohama, Kanagawa, Japan

[3] RIKEN, Inst Phys & Chem Res, RCAI, Lab Epithelial Immunobiol, Yokohama, Kanagawa, Japan

[4] Osaka Univ, Lab Immune Regulat, World Premier Int Res Ctr Initiat WPI Immunol Fro, Osaka, Japan

[5] Osaka Univ, Grad Sch Med, Dept Allergy & Immunol, Osaka, Japan

[6] Emory Univ, Sch Med, Dept Pathol, Atlanta, GA 30322 USA

[7] RIKEN, Plant Sci Ctr, Yokohama, Kanagawa, Japan

[8] Chiba Univ, Grad Sch Med, Dept Med & Clin Oncol K1, Chiba, Japan

[9] Chiba Univ, Grad Sch Med, Dept Immune Regulat, Chiba, Japan

[10] Univ Calif Davis, Sch Vet Med, Dept Anat Physiol & Cell Biol, Ctr Comparat Med, Davis, CA 95616 USA

来源：

NATURE IMMUNOLOGY | 2012年 / 13卷 / 08期

基金：

美国国家卫生研究院; 日本科学技术振兴机构; 比尔及梅琳达.盖茨基金会; 日本学术振兴会;

关键词：

FOLLICLE-ASSOCIATED EPITHELIUM; PEYERS-PATCHES; LYMPHOID FOLLICLES; T-CELLS; STEM-CELLS; MICE; EXPRESSION; INFECTION; PROTEIN; LYMPHOCYTES;

D O I：

10.1038/ni.2352

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Intestinal microfold cells (M cells) are an enigmatic lineage of intestinal epithelial cells that initiate mucosal immune responses through the uptake and transcytosis of luminal antigens. The mechanisms of M-cell differentiation are poorly understood, as the rarity of these cells has hampered analysis. Exogenous administration of the cytokine RANKL can synchronously activate M-cell differentiation in mice. Here we show the Ets transcription factor Spi-B was induced early during M-cell differentiation. Absence of Spi-B silenced the expression of various M-cell markers and prevented the differentiation of M cells in mice. The activation of T cells via an oral route was substantially impaired in the intestine of Spi-B-deficient (Spib(-/-)) mice. Our study demonstrates that commitment to the intestinal M-cell lineage requires Spi-B as a candidate master regulator.

引用

页码：729 / +

页数：10

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