Forty-nine cases of acute lymphoblastic leukaemia/lymphoma in pleural and pericardial effusions: A cytological-histological correlation

被引：8

作者：

Li, J. ^{[1
]}

Zhang, W. ^{[1
]}

Wang, W. ^{[1
]}

Jiang, Y. ^{[1
]}

Zhao, S. ^{[1
]}

Liu, W. ^{[1
]}

Li, G. ^{[1
]}

Liu, N. ^{[1
]}

Li, Q. ^{[1
]}

Su, X. ^{[1
]}

机构：

[1] Sichuan Univ, Dept Pathol, West China Hosp, Chengdu, Sichuan, Peoples R China

来源：

CYTOPATHOLOGY | 2018年 / 29卷 / 02期

关键词：

acute lymphoblastic leukaemia; lymphoma; immunocytochemistry; pericardial effusion; pleural effusion; polymerase chain reaction; DEOXYNUCLEOTIDYL TRANSFERASE TDT; FLOW-CYTOMETRY; GENE REARRANGEMENTS; MALIGNANT-LYMPHOMAS; PARAFFIN SECTIONS; DIAGNOSIS; LEUKEMIA/LYMPHOMA; LEUKEMIA; THYMOMA;

D O I：

10.1111/cyt.12515

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

IntroductionAcute lymphoblastic leukaemia/lymphoma (ALL/LBL) is an aggressive entity of precursor lymphoid neoplasm and may cause malignant serous effusion (SE). The current study aimed to analyse the characteristics of SE cytology of ALL/LBL including cytomorphology, immunophenotyping, clonality and evaluate the effectiveness of SE cytology as a diagnostic method for ALL/LBL. MethodsSE specimens with final diagnosis of ALL/LBL from 2006 to 2016 were reviewed for clinical data, cytomorphological features and ancillary studies. Cytodiagnoses were compared with histodiagnoses, and the discordant cases were analysed. ResultsA total of 49 specimens including 47 pleural fluids and 2 pericardial fluids from 49 patients were evaluated. Cytomorphology revealed lymphoblasts varied from small size with scant cytoplasm, condensed nuclear chromatin and indistinct nucleoli to large size with dispersed nuclear chromatin and multiple variably prominent nucleoli. Nuclear clefts and hand mirror-shaped blasts were demonstrated. The positive rates of CD99 and terminal deoxynucleotidyl transferase were 90.9% and 81.6%, respectively. Both monoclonal immunoglobulin (Ig)H and T-cell receptor- gene rearrangements were demonstrated in 1 of 3 cases. Monoclonal T-cell receptor- gene rearrangement was found in 10 of 11 cases. Monoclonal IgH and/or Ig? gene rearrangements were revealed in 2 of 3 cases. Cytodiagnoses included 4 ALL/LBL, 3 B-ALL/LBL and 42 T-ALL/LBL. Histodiagnoses were available in 24 cases including 2 ALL/LBL, 2 B-ALL/LBL and 20 T-ALL/LBL. The concordance rates of cytological-histological diagnoses were 66.7%, 0% and 95.2% in the three categories, respectively. There were 3 cases with discrepancies of cell lineages. ConclusionsSE cytological evaluation is a reliable and effective method for the diagnosis of ALL/LBL. The current study aimed to analyze the characteristics of cytomorphology, immunophenotyping and clonality to evaluate the effectiveness of serous effusion cytology as a diagnostic method for ALL/LBL.

引用

页码：172 / 178

页数：7

共 20 条

[1] Lymphoblastic lymphoma with unusual cytological features in pericardial effusion fluid [J].

AbdullGaffar, B. ;

AlAbsi, N. .

CYTOPATHOLOGY, 2012, 23 (01) :65-67

[2] Precursor T-Lymphoblastic Lymphoma: Speedy Diagnosis in FNA and Effusion Cytology by Morphology, Immunochemistry, and Flow Cytometry [J].

Bhaker, Poonam ;

Das, Anirban ;

Rajwanshi, Arvind ;

Gautam, Upasana ;

Trehan, Amita ;

Bansal, Deepak ;

Varma, Neelam ;

Srinivasan, Radhika .

CANCER CYTOPATHOLOGY, 2015, 123 (09) :557-565

[3]

Borowitz M.J., 2008, WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues, P168

[4] Pleural effusions in lymphoblastic lymphoma: A diagnostic alternative [J].

Chaignaud, BE ;

Bonsack, TA ;

Kozakewich, HP ;

Shamberger, RC .

JOURNAL OF PEDIATRIC SURGERY, 1998, 33 (09) :1355-1357

[5] Cytological Diagnosis of T Lymphoblastic Leukemia/Lymphoma in Patients with Pleural Effusion as an Initial Clinical Presentation: Two Case Reports of an Algorithmic Approach Using Complete Immunohistochemical Phenotyping [J].

Chen, Lan ;

Zhang, Jin-Song ;

Di Cui ;

Liu, Dong-Ge .

ACTA CYTOLOGICA, 2015, 59 (06) :485-492

[6] Flow cytometry as an adjunct to cytomorphologic serous effusions [J].

Czader, M ;

Ali, SZ .

DIAGNOSTIC CYTOPATHOLOGY, 2003, 29 (02) :74-78

[7] Fine-needle aspiration biopsy in the diagnosis and classification of primary and recurrent lymphoma: A retrospective analysis of the utility of cytomorphology and flow cytometry [J].

Dong, HY ;

Harris, NL ;

Preffer, FI ;

Pitman, MB .

MODERN PATHOLOGY, 2001, 14 (05) :472-481

[8] Flow cytometry in the diagnosis of mediastinal tumors with emphasis on differentiating thymocytes from precursor T-lymphoblastic lymphoma/leukemia [J].

Gorczyca, W ;

Tugulea, S ;

Liu, Z ;

Li, XY ;

Wong, JYL ;

Weisberger, J .

LEUKEMIA & LYMPHOMA, 2004, 45 (03) :529-538

[9] Pitfall in the use of terminal deoxynucleotidyl transferase (TdT) in the cytological diagnosis of lymphoma [J].

Ingoldsby, Helen ;

Billett, Patricia ;

Leader, Mary .

HISTOPATHOLOGY, 2013, 62 (02) :359-360

[10] NOTCH1 Intracellular Domain Immunohistochemistry as a Diagnostic Tool to Distinguish T-Lymphoblastic Lymphoma From Thymoma [J].

Jegalian, Armin G. ;

Bodo, Juraj ;

Hsi, Eric D. .

AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 2015, 39 (04) :565-572

← 1 2 →