Introducing pulsed low-intensity ultrasound to culturing human umbilical cord-derived mesenchymal stem cells

被引：46

作者：

Yoon, Jong Hyun ^{[1
]}

Roh, Eun Youn ^{[1
]}

Shin, Sue ^{[1
]}

Jung, Nam Hee ^{[2
]}

Song, Eun Young ^{[3
]}

Lee, Dong Soon ^{[3
]}

Han, Kyou Sup ^{[3
]}

Kim, Joung Sung ^{[4
]}

Kim, Byoung Jae ^{[5
]}

Jeon, Hye Won ^{[5
]}

Yoon, Kang Sup ^{[6
]}

机构：

[1] Seoul Natl Univ, Boramae Hosp, Dept Lab Med, Seoul 156707, South Korea

[2] Seoul Metropolitan Publ Cord Blood Bank Allcord, Seoul 156707, South Korea

[3] Seoul Natl Univ, Coll Med, Dept Lab Med, Seoul 110744, South Korea

[4] Seoul Natl Univ, Boramae Hosp, Dept Family Med, Seoul 156707, South Korea

[5] Seoul Natl Univ, Boramae Hosp, Dept Obstet & Gynecol, Seoul 156707, South Korea

[6] Seoul Natl Univ, Boramae Hosp, Dept Orthoped Surg, Seoul 156707, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 03期

关键词：

Enzyme digestion; Growth factor; Human umbilical cord; Mesenchymal stem cells; Pulsed low-intensity ultrasound; VEIN;

D O I：

10.1007/s10529-008-9872-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The human umbilical cord (hUC) is a source of adult tissue-derived mesenchymal stem cells (MSCs). A pulsed low-intensity ultrasound (PLIUS) method is described for increasing the yield of MSCs from whole hUC without enzymatic digestion or growth factor supplementation. Analysis of the immunophenotype of cells and a differentiation study were performed to show the compatibility of MSCs. The mean number of cells recovered from primocultures of hUC was 6 x 10(5) cells/cm. PLIUS resulted in a 3.3-fold increase in MSC yield at passage 0. PLIUS exposure increases the yield of hUC-MSCs by promoting release and enhancing proliferation.

引用

页码：CP15 / 335

页数：7

共 15 条

[1] Isolation and culture of umbilical vein mesenchymal stem cells [J].

Covas, DT ;

Siufi, JLC ;

Silva, ARL ;

Orellana, MD .

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2003, 36 (09) :1179-1183

[2] In vitro effects of therapeutic ultrasound on cell proliferation, protein synthesis, and cytokine production by human fibroblasts, osteoblasts, and monocytes [J].

Doan, N ;

Reher, P ;

Meghji, S ;

Harris, M .

JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY, 1999, 57 (04) :409-419

[3] Mesenchymal progenitor cells in human umbilical cord blood [J].

Erices, A ;

Conget, P ;

Minguell, JJ .

BRITISH JOURNAL OF HAEMATOLOGY, 2000, 109 (01) :235-242

[4] Biology of stem cells in human umbilical cord stroma:: In situ and in vitro surveys [J].

Karahuseyinoglu, Sercin ;

Cinar, Ozgur ;

Kilic, Emine ;

Kara, Fadil ;

Akay, Guvem Gumus ;

Demiralp, Duygu Ozel ;

Tukun, Ajlan ;

Uckan, Duygu ;

Can, Alp .

STEM CELLS, 2007, 25 (02) :319-331

[5]

Lu LL, 2006, HAEMATOLOGICA, V91, P1017

[6] Expansion of mesenchymal stem cell isolated from pediatric and adult donor bone marrow [J].

Mareschi, K ;

Ferrero, I ;

Rustichelli, D ;

Aschero, S ;

Gammaitoni, L ;

Aglietta, M ;

Madon, E ;

Fagioli, F .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2006, 97 (04) :744-754

[7] Effects of low-intensity ultrasound (LIUS) stimulation on human cartilage explants [J].

Min, B. -H ;

Woo, J. -I ;

Cho, H. -S ;

Choi, B. H. ;

Park, S. -J ;

Choi, M. J. ;

Park, S. R. .

SCANDINAVIAN JOURNAL OF RHEUMATOLOGY, 2006, 35 (04) :305-311

[8] Matrix cells from Wharton's jelly form neurons and glia [J].

Mitchell, KE ;

Weiss, ML ;

Mitchell, BM ;

Martin, P ;

Davis, D ;

Morales, L ;

Helwig, B ;

Beerenstrauch, M ;

Abou-Easa, K ;

Hildreth, T ;

Troyer, D .

STEM CELLS, 2003, 21 (01) :50-60

[9] Stromal differentiation and architecture of the human umbilical cord [J].

Nanaev, AK ;

Kohnen, G ;

Milovanov, AP ;

Domogatsky, SP ;

Kaufman, P .

PLACENTA, 1997, 18 (01) :53-64

[10] Comparison of gene expression of umbilical cord vein and bone marrow-derived mesenchymal stem cells [J].

Panepucci, RA ;

Siufi, JLC ;

Silva, WA ;

Proto-Siquiera, R ;

Neder, L ;

Orellana, M ;

Rocha, V ;

Covas, DT ;

Zago, MA .

STEM CELLS, 2004, 22 (07) :1263-1278

← 1 2 →