Insulin Induces C2C12 Cell Proliferation and Apoptosis Through Regulation of Cyclin D1 and BAD Expression

被引：13

作者：

Xiong, Cheng-Juan ^{[1
]}

Li, Ping-Fa ^{[2
]}

Song, Yang-Liu ^{[1
]}

Xue, Li-Xiang ^{[3
]}

Jia, Zhu-Qing ^{[3
]}

Yao, Chun-Xia ^{[1
]}

Wei, Qing-Xia ^{[1
]}

Zhang, Shu-Feng ^{[4
]}

Zhang, Shan-Feng ^{[1
]}

Zhang, Yan-Yan ^{[5
]}

Zhao, Ji-Min ^{[1
]}

Wang, Tian-Qi ^{[1
]}

Guo, Mao-Feng ^{[1
]}

Zang, Ming-Xi ^{[1
]}

机构：

[1] Zhengzhou Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Zhengzhou 450001, Henan, Peoples R China

[2] Xinxiang Med Univ, Dept Lab Med, Xinxiang City 453003, Henan, Peoples R China

[3] Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China

[4] Zhengzhou Univ, Peoples Hosp Henan Prov, Zhengzhou 450001, Henan, Peoples R China

[5] Zhengzhou Univ, Sch Pharmaceut Sci, Zhengzhou 450001, Henan, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2013年 / 114卷 / 12期

基金：

中国国家自然科学基金;

关键词：

INSULIN; CELL; GROWTH; APOPTOSIS; CYCLIN D1; BAD; PPAR-ALPHA; CARDIOMYOCYTE PROLIFERATION; ESTROGEN-RECEPTOR; ACTIVATION; GROWTH; DIFFERENTIATION; LOOP;

D O I：

10.1002/jcb.24619

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Insulin is a secreted peptide hormone identified in human pancreas to promote glucose utilization. Insulin has been observed to induce cell proliferation and myogenesis in C2C12 cells. The precise mechanisms underlying the proliferation of C2C12 cells induced by insulin remain unclear. In this study, we observed for the first time that 10nM insulin treatment promotes C2C12 cell proliferation. Additionally, 50 and 100nM insulin treatment induces C2C12 cell apoptosis. By utilizing real-time PCR and Western blotting analysis, we found that the mRNA levels of cyclinD1 and BAD are induced upon 10 and 50nM/100nM insulin treatment, respectively. The similar results were observed in C2C12 cells expressing GATA-6 or PPAR. Our results identify for the first time the downstream targets of insulin, cyclin D1, and BAD, elucidate a new molecular mechanism of insulin in promoting cell proliferation and apoptosis. J. Cell. Biochem. 114: 2708-2717, 2013. (c) 2013 Wiley Periodicals, Inc.

引用

页码：2708 / 2717

页数：10

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