Identification of a highly virulent strain of Xanthomonas axonopodis pv. malvacearum

被引：9

作者：

Huang, Xi ^{[1
,2
]}

Zhai, Jinling ^{[1
]}

Luo, Yuehua ^{[1
]}

Rudolph, Klaus ^{[3
]}

机构：

[1] Hainan Univ, Inst Biol Sci & Technol, Haikou 570228, Peoples R China

[2] Hainan Univ, Key Lab Trop Biol Resources, Minist Educ, Haikou 570228, Peoples R China

[3] Univ Gottingen, Div Plant Pathol & Crop Protect, Fac Agr Sci, D-37077 Gottingen, Germany

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2008年 / 122卷 / 04期

关键词：

Cellulase; Lipopolysaccharide; Rep-PCR;

D O I：

10.1007/s10658-008-9312-3

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A highly virulent strain (HVS) of Xanthomonas axonopodis pv. malvacearum (Xam) was first reported in Africa in 1983, infecting all commercial cultivars of cotton including the immune cv. '101-102B'. The HVS was considered to be a new race of pathovar malvacearum (race 20). Here we studied a HVS (GSPB 2388) isolated in Sudan, which causes symptoms that clearly differed from the typical angular water-soaked spots of bacterial bright of cotton. Our investigations showed that extracellular cellulase activity of this HVS was higher than that of the control strain GSPB 1386 (race 18). Additionally, SDS-PAGE indicated that the HVS cell wall contained short LPS molecules with fewer O-chain repeating units, lacking in GSPB 1386. The higher cellulase activity and the distinct lipopolysaccharide of HVS are correlated with the higher virulence and deviating symptom formation. Rep-PCR fingerprinting showed that the HVS was very closely related to other strains of Xam.

引用

页码：461 / 469

页数：9

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