Nanostructural Response of Mitomycin C Application on Human Scleral Tissues

被引:5
作者
Choi, Samjin [1 ,2 ]
Lee, Seung-Chan [3 ]
Cheong, Youjin [1 ,2 ]
Shin, Jae-Ho [4 ]
Jin, Kyung-Hyun [4 ]
Park, Hun-Kuk [1 ,2 ,5 ]
机构
[1] Kyung Hee Univ, Coll Med, Dept Biomed Engn, Seoul 130701, South Korea
[2] Kyung Hee Univ, Coll Med, Healthcare Ind Res Inst, Seoul 130701, South Korea
[3] Kangwon Natl Univ, Coll Med, Dept Ophthalmol, Kangwon Do 200701, South Korea
[4] Kyung Hee Univ, Coll Med, Dept Ophthalmol, Seoul 130701, South Korea
[5] Kyung Hee Univ, Program Med Engn, Seoul 130701, South Korea
关键词
0.02% MMC Application; Human Scleral Collagen Fibrils; Fibril Diameter and D-Periodicity; Nanostructure; AFM; STRABISMUS SURGERY;
D O I
10.1166/jbn.2013.1510
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
This study quantitatively examined short-term effects of 0.02% Mitomycin C (MMC) treatment on the nanostructural changes in human scleral collagen fibrils. Histologic analysis and non-contact mode atomic force microscopy (AFM) were employed to assess the ultrastructural changes in the morphological characteristics of human sclera before and after 0.02% MMC application for 1 and 3 min. The scleral collagen fibrils treated with 0.02% MMC for 1 min showed no significant change in the morphology of collagen fibrils, and a significant change (p < 0.05) in the thickness of scleral tissues and collagen density, compared to the controls. 0.02% MMC application for 3 min led to a significant increase (p < 0.001) in the mean fibril diameter (185.43 +/- 22.64 nm vs. 140.72 +/- 18.06 nm), thickness (0.81 +/- 0.03 mm vs. 0.54 +/- 0.05 mm) and collagen density (1.16 times), compared to the controls This study examined the nanostructural changes in the scleral collagen fibrils before and after MMC application by AFM technique combined with conventional histological analysis (Hematoxylin-eosin and Masson's trichrome). This result indirectly suggests that long-term MMC application might increase the incidence of complications like a scleromalcia.
引用
收藏
页码:1393 / 1397
页数:5
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