Enantiomeric separation of five amino acids by high-performance liquid chromatography on a chiral crown ether column

被引：0

作者：

Gimenez, F ^{[1
]}

Soursac, M ^{[1
]}

Farinotti, R ^{[1
]}

机构：

[1] UNIV PARIS SUD,FAC PHARM,DEPT CLIN PHARM,CHATENAY MALABR,FRANCE

来源：

CHIRALITY | 1997年 / 9卷 / 02期

关键词：

amino acid; stereoconversion; racemization; liquid chromatography; isomer;

D O I：

10.1002/(SICI)1520-636X(1997)9:2<150::AID-CHIR13>3.0.CO;2-M

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A chiral liquid chromatographic method was validated to analyze the D- and L-enantiomers of five amino acids contained in a commercial solution: aspartic acid, leucine, lysine, phenylalanine, and valine. These 10 compounds were separated on a chiral crown ether column with a mobile phase composed of water adjusted to pH 1.5 with perchloric acid, with ultraviolet detection at 220 nm. The method was applied to the commercial amino acid solution before and after sterilization by 5 kGy irradiation; no stereoconversion was observed following sterilization. (C) 1997 Wiley-Liss, Inc.

引用

页码：150 / 152

页数：3