Clinical isolates of Yersinia enterocolitica Biotype 1A represent two phylogenetic lineages with differing pathogenicity-related properties

被引:34
作者
Sihvonen, Leila M. [1 ]
Jalkanen, Kaisa [1 ]
Huovinen, Elisa [2 ]
Toivonen, Susanna [3 ]
Corander, Jukka [4 ]
Kuusi, Markku [2 ]
Skurnik, Mikael [3 ,5 ]
Siitonen, Anja [1 ]
Haukka, Kaisa [1 ,6 ]
机构
[1] Natl Inst Hlth & Welf THL, Bacteriol Unit, Helsinki, Finland
[2] Natl Inst Hlth & Welf THL, Epidemiol Surveillance & Response Unit, Helsinki, Finland
[3] Univ Helsinki, Dept Bacteriol & Immunol, Haartman Inst, Helsinki, Finland
[4] Univ Helsinki, Dept Math & Stat, Helsinki, Finland
[5] Univ Helsinki, Cent Hosp, Diagnost Lab, Helsinki, Finland
[6] Univ Helsinki, Dept Food & Environm Sci, Helsinki, Finland
关键词
Yersinia enterocolitica biotype 1A; MLST; 16S rRNA gene; yst genes; LPS; Phage typing; Human serum complement killing; Bayesian analysis of population structure; Pathogenicity; GENETIC-RELATIONSHIPS; POPULATION-STRUCTURE; CLONAL GROUPS; BIOVAR; 1A; FACTOR-H; STRAINS; LIPOPOLYSACCHARIDE; SEQUENCE; IDENTIFICATION; YST;
D O I
10.1186/1471-2180-12-208
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Y. enterocolitica biotype (BT) 1A strains are often isolated from human clinical samples but their contribution to disease has remained a controversial topic. Variation and the population structure among the clinical Y. enterocolitica BT 1A isolates have been poorly characterized. We used multi-locus sequence typing (MLST), 16S rRNA gene sequencing, PCR for ystA and ystB, lipopolysaccharide analysis, phage typing, human serum complement killing assay and analysis of the symptoms of the patients to characterize 298 clinical Y. enterocolitica BT 1A isolates in order to evaluate their relatedness and pathogenic potential. Results: A subset of 71 BT 1A strains, selected based on their varying LPS patterns, were subjected to detailed genetic analyses. The MLST on seven house-keeping genes (adk, argA, aroA, glnA, gyrB, thrA, trpE) conducted on 43 of the strains discriminated them into 39 MLST-types. By Bayesian analysis of the population structure (BAPS) the strains clustered conclusively into two distinct lineages, i.e. Genetic groups 1 and 2. The strains of Genetic group 1 were more closely related (97% similarity) to the pathogenic bio/serotype 4/O:3 strains than Genetic group 2 strains (95% similarity). Further comparison of the 16S rRNA genes of the BT 1A strains indicated that altogether 17 of the 71 strains belong to Genetic group 2. On the 16S rRNA analysis, these 17 strains were only 98% similar to the previously identified subspecies of Y. enterocolitica. The strains of Genetic group 2 were uniform in their pathogenecity-related properties: they lacked the ystB gene, belonged to the same LPS subtype or were of rough type, were all resistant to the five tested yersiniophages, were largely resistant to serum complement and did not ferment fucose. The 54 strains in Genetic group 1 showed much more variation in these properties. The most commonly detected LPS types were similar to the LPS types of reference strains with serotypes O:6,30 and O:6,31 (37%), O:7,8 (19%) and O:5 (15%). Conclusions: The results of the present study strengthen the assertion that strains classified as Y. enterocolitica BT 1A represent more than one subspecies. Especially the BT 1A strains in our Genetic group 2 commonly showed resistance to human serum complement killing, which may indicate pathogenic potential for these strains. However, their virulence mechanisms remain unknown.
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共 63 条
[1]   THE EFFECT OF GROWTH TEMPERATURE ON THE BIOSYNTHESIS OF YERSINIA-ENTEROCOLITICA 0-3 LIPOPOLYSACCHARIDE - TEMPERATURE REGULATES THE TRANSCRIPTION OF THE RFB BUT NOT OF THE RFA REGION [J].
ALHENDY, A ;
TOIVANEN, P ;
SKURNIK, M .
MICROBIAL PATHOGENESIS, 1991, 10 (01) :81-86
[2]   The pathogenic potential of Yersinia enterocolitica 1A [J].
Batzilla, Julia ;
Heesemann, Juergen ;
Rakin, Alexander .
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 301 (07) :556-561
[3]   Regulatory network of lipopolysaccharide O-antigen biosynthesis in Yersinia enterocolitica includes cell envelope-dependent signals [J].
Bengoechea, JA ;
Zhang, LH ;
Toivanen, P ;
Skurnik, M .
MOLECULAR MICROBIOLOGY, 2002, 44 (04) :1045-1062
[4]   Distribution of virulence-associated genes in Yersinia enterocolitica biovar 1A correlates with clonal groups and not the source of isolation [J].
Bhagat, Neeru ;
Virdi, Jugsharan S. .
FEMS MICROBIOLOGY LETTERS, 2007, 266 (02) :177-183
[5]   The Enigma of Yersinia enterocolitica biovar 1A [J].
Bhagat, Neeru ;
Virdi, Jugsharan S. .
CRITICAL REVIEWS IN MICROBIOLOGY, 2011, 37 (01) :25-39
[6]   Role of YadA, Ail, and lipopolysaccharide in serum resistance of Yersinia enterocolitica serotype O:3 [J].
Biedzka-Sarek, M ;
Venho, R ;
Skurnik, M .
INFECTION AND IMMUNITY, 2005, 73 (04) :2232-2244
[7]   Functional Mapping of YadA- and Ail-Mediated Binding of Human Factor H to Yersinia enterocolitica Serotype O:3 [J].
Biedzka-Sarek, Marta ;
Salmenlinna, Saara ;
Gruber, Markus ;
Lupas, Andrei N. ;
Meri, Seppo ;
Skurnik, Mikael .
INFECTION AND IMMUNITY, 2008, 76 (11) :5016-5027
[8]   Characterization of complement factor H binding to Yersinia enterocolitica serotype O:3 [J].
Biedzka-Sarek, Marta ;
Jarva, Hanna ;
Hyytiainen, Heidi ;
Meri, Seppo ;
Skurnik, Mikael .
INFECTION AND IMMUNITY, 2008, 76 (09) :4100-4109
[9]   Association between clinical presentation, biogroups and virulence attributes of Yersinia enterocolitica strains in human diarrhoeal disease [J].
Burnens, AP ;
Frey, A ;
Nicolet, J .
EPIDEMIOLOGY AND INFECTION, 1996, 116 (01) :27-34
[10]   Bayesian analysis of population structure based on linked molecular information [J].
Corander, Jukka ;
Tang, Jing .
MATHEMATICAL BIOSCIENCES, 2007, 205 (01) :19-31