Fabrication and Electrochemical Characterization of Nanoporous Silicon Electrode for Amperometric Urea Biosensor

被引：8

作者：

Yun, Donghwa ^{[1
]}

Song, Min-Jung ^{[2
]}

Hwang, Sungwoo ^{[2
]}

Hong, Suk-In ^{[1
]}

机构：

[1] Korea Univ, Dept Chem & Biol Engn, Seoul 136701, South Korea

[2] Korea Univ, Sch Elect Engn, Seoul 136701, South Korea

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS | 2012年 / 51卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

POROUS SILICON; IMPEDANCE MEASUREMENT; ENZYME ELECTRODE; GOLD ELECTRODE; TIO2; ELECTRODE; DNA BIOSENSOR; SENSOR; GLUCOSE; CARBON; ACID;

D O I：

10.1143/JJAP.51.06FG02

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We describe a new type of biosensor that employs a modified gold electrode based on nanoporous silicon (NPSi) for the electrochemical detection of urea. Urease (Urs) was covalently immobilized onto an Au/NPSi electrode functionalized with 3-mercaptopropionic acid (3-MPA). Amperometric calibration curves for both NPSi and planar silicon (PLSi)-based urea sensitive electrodes were compared in the range of 0.3 to 4.5 mM urea concentrations. The Michaelis-Menten constant (K-m) was determined using the amperometric method. The electrochemical active area (A(ea)) of the 3-MPA/Au/NPSi electrode was evaluated using cyclic voltammetry (CV) and the result was compared with the 3-MPA/Au/PLSi electrode. Measured sensitivity of the Urs/SAMs/Au/NPSi electrode is ca. 2.05 mu A mM(-1) cm(-2) and that of the Urs/SAMs/Au/PLSi electrode is ca. 1.10 mu A mM(-1) cm(-2). About 1.8 times of sensitivity increase is obtained in the Au/NPSi electrode. (C) 2012 The Japan Society of Applied Physics

引用

页数：4

共 30 条

[1] A photochemical cell with nano-porous TiO2 electrode sensitized by lignophenol under visible light irradiation [J].

Aoyagi, Mitsuru ;

Funaoka, Masamitsu .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2006, 181 (01) :114-119

[2] Macroporous silicon electrical sensor for DNA hybridization detection [J].

Archer, M ;

Christophersen, M ;

Fauchet, PM .

BIOMEDICAL MICRODEVICES, 2004, 6 (03) :203-211

[3] SOLUBLE METALLOENDOPEPTIDASE FROM RAT-BRAIN - ACTION ON ENKEPHALIN-CONTAINING PEPTIDES AND OTHER BIOACTIVE PEPTIDES [J].

CHU, TG ;

ORLOWSKI, M .

ENDOCRINOLOGY, 1985, 116 (04) :1418-1425

[4] The structural and luminescence properties of porous silicon [J].

Cullis, AG ;

Canham, LT ;

Calcott, PDJ .

JOURNAL OF APPLIED PHYSICS, 1997, 82 (03) :909-965

[5] Facile fabrication of a nanoporous silicon electrode with superior stability for lithium ion batteries [J].

Du, Chunyu ;

Gao, Cuihua ;

Yin, Geping ;

Chen, Meng ;

Wang, Long .

ENERGY & ENVIRONMENTAL SCIENCE, 2011, 4 (03) :1037-1042

[6] A UREA-SPECIFIC ENZYME ELECTRODE [J].

GUILBAULT, GG ;

MONTALVO, JG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1969, 91 (08) :2164-+

[7] Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measurement of enzyme-catalyzed polymer transformation [J].

Ho, WO ;

Krause, S ;

McNeil, CJ ;

Pritchard, JA ;

Armstrong, RD ;

Athey, D ;

Rawson, K .

ANALYTICAL CHEMISTRY, 1999, 71 (10) :1940-1946

[8] Electrochemical DNA Biosensor Based on Nanoporous Gold Electrode and Multifunctional Encoded DNA-Au Bio Bar Codes [J].

Hu, Kongcheng ;

Lan, Dongxiao ;

Li, Xuemei ;

Zhang, Shusheng .

ANALYTICAL CHEMISTRY, 2008, 80 (23) :9124-9130

[9] Silver UPD ultra-thin film modified nanoporous gold electrode with applications in the electrochemical detection of chloride [J].

Huang, Jing-Fang .

TALANTA, 2009, 77 (05) :1694-1700

[10] Porous silicon biosensors on the advance [J].

Jane, Andrew ;

Dronov, Roman ;

Hodges, Alastair ;

Voelcker, Nicolas H. .

TRENDS IN BIOTECHNOLOGY, 2009, 27 (04) :230-239

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