A bipolar kinesin

被引:305
作者
Kashina, AS [1 ]
Baskin, RJ [1 ]
Cole, DG [1 ]
Wedaman, KP [1 ]
Saxton, WM [1 ]
Scholey, JM [1 ]
机构
[1] INDIANA UNIV,DEPT BIOL,BLOOMINGTON,IN 47405
关键词
D O I
10.1038/379270a0
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
CHROMOSOME Segregation during mitosis depends on the action of the mitotic spindle, a self-organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors(1,2). Members of the BimC subfamily of kinesin-related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle(3-14). Here we report that KRP(130), a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos(13), has an ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament(15). Such a bipolar 'minifilament' could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.
引用
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页码:270 / 272
页数:3
相关论文
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