Graphene oxide dispersed supramolecular hydrogel capped benign green silver nanoparticles for anticancer, antimicrobial, cell attachment and intracellular imaging applications

Graphene oxide (GO) nanosheets dispersed a supramolecular hydrogel network (GOSHGeI) has been achieved. The microstructures of GO dispersed hydrogels are investigated. Our previously reported a benign supramolecular hydrogel (SHGeI) was used and mixed with exfoliated monolayers of GO to offer GOSHGeI through several interplaying non-covalent type supramolecular interactions. The GOSHGeI was employed as the stabilizing agent for the direct sun-ray mediated photo-chemically synthesized 'Green' silver nanoparticles (Ag-NPs). In vitro and in vivo toxicity assessments approve the bio-relevant nature of the GOSHGel capped Ag-NPs. The Ag-NPs were potentially antimicrobial against bacteria, including gram -ve bacterium (Escherichia coli) and gram +ve bacterium (Bacillus subtilis), and fungal pathogen, Pichia guilliermondii. Remarkably, these GOSHGeI capped Ag-NPs also showed tremendous anticancer activity tested against human breast cancer cell. Both, GOSHGeI and Ag-NPs embedded GOSHGeI, were useful as the cell supportive scaffolds. This cell attachment was selective for non-malignant cell i.e. macrophages. Confocal microscopic study revealed the luminescent nature of GOSHGeI capped Ag-NPs. (C) 2019 Elsevier B.V. All rights reserved.