Nanoflow electrospinning serial femtosecond crystallography

被引：143

作者：

Sierra, Raymond G. ^{[1
]}

Laksmono, Hartawan ^{[1
]}

Kern, Jan ^{[2
,3
]}

Rosalie Tran ^{[2
]}

Hattne, Johan ^{[2
]}

Alonso-Mori, Roberto ^{[3
]}

Lassalle-Kaiser, Benedikt ^{[2
]}

Gloeckner, Carina ^{[4
]}

Hellmich, Julia ^{[4
]}

Schafer, Donald W. ^{[3
]}

Echols, Nathaniel ^{[2
]}

Gildea, Richard J. ^{[2
]}

Grosse-Kunstleve, Ralf W. ^{[2
]}

Sellberg, Jonas ^{[5
,6
]}

McQueen, Trevor A. ^{[7
]}

Fry, Alan R. ^{[3
]}

Messerschmidt, Marc M. ^{[3
]}

Miahnahri, Alan ^{[3
]}

Seibert, M. Marvin ^{[3
]}

Hampton, Christina Y. ^{[1
]}

Starodub, Dmitri ^{[1
]}

Loh, N. Duane ^{[1
]}

Sokaras, Dimosthenis ^{[5
]}

Weng, Tsu-Chien ^{[5
]}

Zwart, Petrus H. ^{[2
]}

Glatzel, Pieter ^{[8
]}

Milathianaki, Despina ^{[3
]}

White, William E. ^{[3
]}

Adams, Paul D. ^{[2
]}

Williams, Garth J. ^{[3
]}

Boutet, Sebastien ^{[3
]}

Zouni, Athina ^{[4
]}

Messinger, Johannes ^{[9
]}

Sauter, Nicholas K. ^{[2
]}

Bergmann, Uwe ^{[3
]}

Yano, Junko ^{[2
]}

Yachandra, Vittal K. ^{[2
]}

Bogan, Michael J. ^{[1
]}

机构：

[1] SLAC Natl Accelerator Lab, PULSE Inst, Menlo Pk, CA 94025 USA

[2] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

[3] SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA

[4] Tech Univ Berlin, Max Volmer Lab Biophys Chem, D-10623 Berlin, Germany

[5] SLAC Natl Accelerator Lab, SSRL, Menlo Pk, CA 94025 USA

[6] Stockholm Univ, Dept Phys, AlbaNova, S-10691 Stockholm, Sweden

[7] Stanford Univ, Dept Chem, Menlo Pk, CA 94025 USA

[8] European Synchrotron Radiat Facil, F-38043 Grenoble, France

[9] Umea Univ, Inst Kemi, Kemiskt Biol Ctr, Umea, Sweden

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2012年 / 68卷

关键词：

PHOTOSYSTEM-II; DIFFRACTION;

D O I：

10.1107/S0907444912038152

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 mu l min(-1) to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 mu l min(-1) and diffracted to beyond 4 angstrom resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 mu g of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.

引用

页码：1584 / 1587

页数：4

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