Evaluating the solid retention time of bacteria in flocculent and granular sludge

被引:83
作者
Winkler, Mari K. H. [1 ]
Kleerebezem, Robbert [1 ]
Khunjar, Wendell O. [2 ]
de Bruin, Bart [3 ]
van Loosdrecht, Mark C. M. [1 ]
机构
[1] Delft Univ Technol, Dept Biotechnol, NL-2628 BC Delft, Netherlands
[2] Hazen & Sawyer PC, Fairfax, VA 22030 USA
[3] DHV, NL-3818 EX Amersfoort, Netherlands
关键词
Aerobic granular sludge; Wash-out; SRT; qPCR; Protozoa; Decay; BIOLOGICAL PHOSPHORUS REMOVAL; GRADIENT GEL-ELECTROPHORESIS; POLYPHOSPHATE-ACCUMULATING ORGANISMS; ACTIVATED-SLUDGE; POPULATION-DYNAMICS; OXIDIZING BACTERIA; LABORATORY-SCALE; RNA; DIVERSITY; COMMUNITY;
D O I
10.1016/j.watres.2012.06.027
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The specific solid retention time for different bacteria within flocculent and granular sludge was determined. Samples were collected from reactor and effluent sludge and the number of a specific bacterial group was evaluated in respect to the total bacterial community with quantitative polymerase chain reaction (qPCR). The ratio of the relative presence of a specific bacterial group in the reactor sludge and wasted sludge was established to observe if preferential wash-out occurred. From the data also the solid retention time for different microbial groups can be estimated. Using this tool, we were able to show that the SRT of populations found on the exterior of granules is slightly lower than the SRT for population in the interior. Archaea were not found in the flocculent system but were present in small amounts within the granular system. It was further observed that protozoa were grazing on the bacterial community within the system indicating that they have the potential to shorten the specific SRT of bacteria. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4973 / 4980
页数:8
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